The availability of sensitive cell lines and molecular methods have allowed for epidemiological surveillance of nonpoliomyelitic enteroviruses in Poland in 2004-2008. The aim of the study was evaluation ofenterovirus diagnosis based on RT-PCR reaction. Available data collected in 2004-2008 indicated that 116 isolation of enteroviruses were performed. The most frequently isolated enterovirus serotype was Coxsackievirus B (30.17%). Other enteroviruses serotypes isolated during study period were Coxsackievirus A (8.62%), ECHO3 (1.72%), ECHO6 (7.76%), ECHO7 (4.31%), ECHO25 (0.86%), ECHO30 (18.97%), poliovirus (0.86%).
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