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A multi-class support vector machine classification model based on 14 microRNAs for forensic body fluid identification.
Forensic Sci Int Genet
February 2025
Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. Electronic address:
Article Synopsis
- MicroRNAs (miRNAs) show promise for identifying body fluids in forensics due to their stability and unique expression patterns, but their relative expression makes identification challenging.
- The study validated reference genes and selected a combination of miRNAs to create a multi-class support vector machine (MSVM) model that predicts body fluid origins with high accuracy, even for aged or mixed samples.
- Further validation across different labs is needed before implementing miRNAs in routine forensic practice.
Longitudinal evaluation of laboratory results and method precision in worldwide erythropoietin external quality assessments.
Front Mol Biosci
June 2024
Faculty of Medical and Life Sciences, Furtwangen University, Villingen-Schwenningen, Germany.
This study presents a longitudinal analysis of external quality assessment (EQA) results for erythropoietin (EPO) determinations conducted between 2017 and 2022 with a continuously increasing number of participating laboratories. The aim of this work was to evaluate participant performance and methodological aspects. In each of the eleven EQA surveys, a blinded sample set of lyophilized human serum containing one sample with lower EPO concentrations (L) and one with higher EPO concentrations (H) was sent to the participating laboratories.
View Article and Find Full Text PDFEstablishment of human post-vaccination SARS-CoV-2 standard reference sera.
J Immunol Methods
July 2024
Iowa City Veterans Administration Healthcare System, 601 Highway 6, Iowa City, IA 52246, USA; The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA. Electronic address:
There is a critical need to understand the effectiveness of serum elicited by different SARS-CoV-2 vaccines against SARS-CoV-2 variants. We describe the generation of reference reagents comprised of post-vaccination sera from recipients of different primary vaccines with or without different vaccine booster regimens in order to allow standardized characterization of SARS-CoV-2 neutralization in vitro. We prepared and pooled serum obtained from donors who received a either primary vaccine series alone, or a vaccination strategy that included primary and boosted immunization using available SARS-CoV-2 mRNA vaccines (BNT162b2, Pfizer and mRNA-1273, Moderna), replication-incompetent adenovirus type 26 vaccine (Ad26.
View Article and Find Full Text PDFTo what extent does freezing impact the mid-infrared signature of urine? Case of patients attending urology department.
Spectrochim Acta A Mol Biomol Spectrosc
March 2024
BioSpecT EA7506 Laboratory, Université de Reims Champagne-Ardenne, Reims, France; Cellular and Tissular Imaging Platform (PICT), Université de Reims Champagne-Ardenne, Reims, France. Electronic address:
Urine is a very interesting and attractive biofluid for biomarker discovery and medical diagnosis research due to its non-invasiveness collection and richness of potential biomarkers. Fourier-Transform Infrared (FTIR) spectroscopy applied on urine samples is a promising tool that could be used as a screening method for various diseases. However, during method development, frozen urine is more accessible, especially for inter-laboratory studies, whereas in routine application fresh urine is more convenient.
View Article and Find Full Text PDFImpact of wet-lab protocols on quality of whole-genome short-read sequences from foodborne microbial pathogens.
Front Microbiol
November 2023
Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
For successful elucidation of a food-borne infection chain, the availability of high-quality sequencing data from suspected microbial contaminants is a prerequisite. Commonly, those investigations are a joint effort undertaken by different laboratories and institutes. To analyze the extent of variability introduced by differing wet-lab procedures on the quality of the sequence data we conducted an interlaboratory study, involving four bacterial pathogens, which account for the majority of food-related bacterial infections: spp.
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