AI Article Synopsis
- The study aimed to investigate early changes in cartilage and bone related to TMJ osteoarthritis by analyzing biglycan/fibromodulin double-deficient mice, which develop the condition by 6 months.
- Researchers used various techniques including micro-CT and electron microscopy on 3-month and 9-month old mice to evaluate bone and cartilage characteristics.
- Results indicated significant early alterations in cartilage structure and gene expression in young double-deficient mice, with distinct later changes in bone in older mice, suggesting that cartilage changes occur before bone changes in TMJ osteoarthritis.
Article Abstract
Objective: Little is known about the natural progression of the disease process of temporomandibular joint (TMJ) osteoarthritis (OA), which affects approximately 1% of the US population. The goal of this study was to examine the early microarchitectural and molecular changes in the condylar cartilage and subchondral bone in biglycan/fibromodulin (Bgn/Fmod) double-deficient mice, which develop TMJ-OA at 6 months.
Methods: TMJs from 3-month-old (n=44) and 9-month-old (n=52) wild-type (WT n=46) and Bgn/Fmod (n=50) double-deficient mice were evaluated. Micro-CT analysis of the subchondral bone (n=24), transmission electron microscopy for condylar cartilage fibril diameters (n=26), and real-time PCR analysis for gene expression for bone and cartilage maturation markers (n=45) was performed.
Results: A statistically significant increase in collagen fibril diameter of the condylar cartilage and a decrease in expression of Parathyroid related protein in the mandibular condylar head were observed in the 3-month Bgn/Fmod double-deficient mice compared to WT controls. The 9-month Bgn/Fmod double-deficient mouse demonstrated an increase in bone volume and total volume in subchondral bone, and an increase in the expression of Collagen Type X and Aggrecan in the mandibular condylar head compared to the WT controls.
Conclusion: We found that changes in the microarchitecture of the condylar cartilage preceded changes in the subchondral bone during OA in the TMJ in Bgn/Fmod double-deficient mice.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787630 | PMC |
| http://dx.doi.org/10.1016/j.archoralbio.2009.10.001 | DOI Listing |
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