AI Article Synopsis
- The objective was to create transgenic Pinellia ternata plants that are resistant to fungal attacks by incorporating chitinase and beta-1,3-glucanase genes from Trichoderma harzianum.
- The method involved using Agrobacterium-mediated transformation to transfer the genes into P. ternata callus, with hygromycin phosphotransferase serving as a selection marker.
- Results confirmed successful integration of the genes into transgenic lines, with stable inheritance observed up to the T5 generation, indicating the effectiveness of the gene transfer.
Article Abstract
Objective: To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.
Method: Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.
Result: PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.
Conclusion: The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata
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