[Study on DNA damage in L-02 cell induced by acrylamide].

Objective: To investigate the cell cycle, cell apoptosis and DNA damage induced by acrylamide (AA) in L-02 human liver embryo cell line.

Methods: The rate of cell survivors, cell cycle, cell apoptosis and DNA damage were measured respectively after L-02 cell were exposed to 0.01, 0.1, 0.5, 1.0, 2.5, 5.0 and 7.5 mmol/L AA for 12h and 24h in vitro.

Results: The rates of cellular survivors in the low (0.01 mmol/L and 0.1 mmol/) dose AA-treated group were higher than those in control group, but those in the middle (0.5, 1.0 and 2.5 mmol/L) and high (5.0 mmol/L and 7.5 mmol/L) dose AA-treated groups were significantly lower than those in control group (P < 0.05). The rate of cell in G1 stage was reduced along with the promotion of AA, and the middle and high (> 1.0 mmol/L) dose AA-treated groups were significantly lower than the control group (P < 0.05), while the rate of cell in S stage was in reverse. The percentages of apoptosis were significantly higher than that of the control group in the high dose AA-treated groups (P < 0.05). The percents of tail DNA and Oliver Tail Moment (OTM) were significantly higher than that of the control group in the middle and high (> 1.0 mmol/L) dose AA-treated groups (P < 0.05).

Conclusion: AA could induce the confusion and apoptosis of L-02 cell, and has impairment effect on DNA.