AI Article Synopsis
- Measuring carboxyhemoglobin (COHb) levels is important for confirming carbon monoxide exposure in forensic and occupational contexts.
- A common method uses spectrophotometric readings, but it excels only at low (1-7%) or high (>20%) COHb concentrations, often failing at middle ranges.
- The authors suggest recalibrating the measurement factors (F1, F2, F3) for each test to ensure better accuracy and precision in COHb results.
Article Abstract
The measurement of carboxyhemoglobin (COHb) levels in blood is a valuable procedure to confirm exposure to carbon monoxide (CO) either for forensic or occupational matters. A previously described method using spectrophotometric readings at 420 and 432 nm after reduction of oxyhemoglobin (O(2)Hb) and methemoglobin with sodium hydrosulfite solution leads to an exponential curve. This curve, used with pre-established factors, serves well for lower concentrations (1-7%) or for high concentrations (> 20%) but very rarely for both. The authors have observed that small variations on the previously described factors F1, F2, and F3, obtained from readings for 100% COHb and 100% O(2)Hb, turn into significant changes in COHb% results and propose that these factors should be determined every time COHb is measured by reading CO and O(2) saturated samples. This practice leads to an increase in accuracy and precision.
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| http://dx.doi.org/10.1093/jat/33.8.540 | DOI Listing |
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