1. The globin prepared from hemoglobin by the acid acetone method is denatured globin. 2. The denaturation and coagulation of globin by acid acetone are reversible. 3. Soluble globin can be obtained from the acid acetone globin even if the globin is first precipitated by trichloracetic acid or heated to 100 degrees C. 4. Hill and Holden's theory that they separated native globin from hemoglobin without any intermediate denaturation is not proven by their experiments.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141132 | PMC |
| http://dx.doi.org/10.1085/jgp.14.5.605 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural and Kinetic Characterization of an Acetoacetyl-Coenzyme A: Acetate Coenzyme A Transferase from the Extreme Thermophile Thermosipho melanesiensis.
Biochem J
January 2025
North Carolina State University, Raleigh, North Carolina, United States.
CtfAB from the extremely thermophilic bacterium, Thermosipho melanesiensis, has been used for in vivo acetone production up to 70°C. This enzyme has tentatively been identified as the rate-limiting step, due to its relatively low binding affinity for acetate. However, existing kinetic and mechanistic studies on this enzyme are insufficient to evaluate this hypothesis.
View Article and Find Full Text PDFFrom solubility to efficiency: Per- and polyfluoroalkyl substances (PFAS) regeneration from anion exchange resins.
Sci Total Environ
January 2025
Department of Civil and Environmental Engineering, Temple University, Philadelphia, PA 19122, USA. Electronic address:
This study investigated the regenerability of anion exchange resins for per- and polyfluoroalkyl substances (PFAS), focusing on the interaction between regenerant composition and resin characteristics. The influence of salt type and concentration on PFAS solubility revealed a general decline in perfluorohexane sulfonate (PFHxS) solubility with increased salt concentrations, most strongly with KCl followed by NaCl and NHCl. Mixed solubility results were observed for perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS).
View Article and Find Full Text PDFThe Synthesis, Crystal Structure, Modification, and Cytotoxic Activity of α-Hydroxy-Alkylphosphonates.
Molecules
January 2025
Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., 1111 Budapest, Hungary.
A series of α-hydroxy-alkylphosphonates and α-hydroxy-alkylphosphine oxides were synthesized by the Pudovik reaction of acetaldehyde and acetone with dialkyl phosphites or diarylphosphine oxides. The additions were performed in three different ways: in liquid phase using triethylamine as the catalyst (1), on the surface of AlO/KF solid catalyst (2), or by a MW-assisted NaCO-catalyzed procedure (3). In most of the cases, our methods were more efficient and more robust than those applied in the literature.
View Article and Find Full Text PDFQualitative Phytochemical Profiling and In Vitro Antioxidant Potential Evaluation of South African Momordica Balsamina Linn Fruit Pulp.
Life (Basel)
December 2024
Division of Pharmacology, Faculty of Pharmacy, Rhodes University, P.O. Box 94, Grahamstown 6139, South Africa.
Linn is a well-known African traditional herb due to its tremendous medicinal and nutritional properties. It is used worldwide for the treatment of different ailments and diseases. In the present study, the phytochemical and antioxidant activity of South African fruit pulp extracts was evaluated.
View Article and Find Full Text PDFCombination of HSP90 Inhibitors and HSP70 Inducers Prevent Hydrochloric Acid-Induced Pulmonary Fibrosis in Rabbits.
Int J Mol Sci
January 2025
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Combined therapies with Heat Shock Protein 90 (HSP90) inhibitors and Heat Shock Protein 70 (HSP70) inducers are gaining significant interest in cancer and cardiovascular research. Here, we tested the hypothesis that HSP90 inhibitors and HSP70 inducers, together, can block the development of pulmonary fibrosis. We exposed New Zealand White Rabbits to hydrochloric acid (HCl, 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!