Müeller-Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus neoformans and Cryptococcus gattii to posaconazole with Etest.

Mycoses

Infectious Disease and Mycology Research Unit. Institut Municipal d 'Investigació Mèdica, Parc de Recerca Biomèdica de Barcelona, Barcelona, Spain.

Published: March 2010

Mueller-Hinton modified agar (MH-GMB) was compared with RPMI + 2% glucose-agar to determine the MICs of 80 isolates of Cryptococcus neoformans and C. gattii to posaconazole with Etest. MH-GMB minimised trailing and agreement between both media was 94%. Agreement of M27-A2 microbroth reference method was 98% with RPMI and 94% with MB-GMB.

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http://dx.doi.org/10.1111/j.1439-0507.2008.01678.xDOI Listing

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