AI Article Synopsis
- The study investigated how soluble total proteins from Zaocys dhumnades affect certain inflammatory markers in human fibroblast-like synoviocytes derived from rheumatoid arthritis patients.
- Different concentrations of these proteins were tested alongside known controls, using techniques like ELISA and RT-PCR to measure the levels of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and IL-10.
- Results showed that higher concentrations of the proteins reduced levels of IL-1beta and TNF-alpha while increasing IL-10, suggesting a potential therapeutic effect against rheumatoid arthritis.
Article Abstract
Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro.
Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect the expressions of IL-1beta, IL-10 and TNF-alpha in the FLS.
Results: The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01).
Conclusion: The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS.
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