The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, on the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/la903068v | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel aggregation-induced emission-featured hyperbranched poly(amido amine)s stabilized copper nanoclusters‑cerium (III) sensor for detection of thiol flavor compounds in processed meat.
Food Chem
February 2025
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Thiol flavor compounds are a class of flavoring ingredients that contribute significantly to food flavor. However, rapid discrimination of multiple thiol-flavor compounds remain a challenge. In this study, a ratiometric fluorescent sensor (TPE-ssHPA@Cu NCs-Ce) with dual-channel fluorescence features was developed using tetraphenylethene-embedded hyperbranched poly(amidoamine) as a template to stabilize the copper nanocluster‑cerium ions.
View Article and Find Full Text PDFBiological Response Following the Systemic Injection of PEG-PAMAM-Rhodamine Conjugates in Zebrafish.
Pharmaceutics
April 2024
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal.
Numerous therapeutic and diagnostic approaches used within a clinical setting depend on the administration of compounds via systemic delivery. Biomaterials at the nanometer scale, as dendrimers, act as delivery systems by improving cargo bioavailability, circulation time, and the targeting of specific tissues. Although evaluating the efficacy of pharmacological agents based on nanobiomaterials is crucial, conducting toxicological assessments of biomaterials is essential for advancing clinical translation.
View Article and Find Full Text PDFA hydrogel-based ratiometric fluorescent sensor relying on rhodamine B labelled AIE-featured hyperbranched poly(amido amine) for heparin detection.
Anal Chim Acta
April 2024
Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Sciences, Tianjin University, Tianjin, 300354, China. Electronic address:
Article Synopsis
- This research focuses on developing a flexible fluorescent sensor (TR-HPA) for detecting Heparin, an important anticoagulant drug, using a hyperbranched poly(amido amine) material enhanced with fluorescent dyes.
- The sensor works by showing a change in fluorescence at two different wavelengths when exposed to Heparin, allowing for a precise measurement of the drug's concentration within a range of 0-1.0 μM, with a very low detection limit of 3.0 nM.
- When combined with a polyvinyl alcohol (PVA) hydrogel, the TR-HPA sensor creates a simple, visual method for Heparin detection that changes color
Polyamidoamine dendrimer decorated graphene oxide as a pH-sensitive nanocarrier for the delivery of hydrophobic anticancer drug quercetin: a remedy for breast cancer.
J Pharm Pharmacol
June 2023
Prof. Rajendra Singh Nanoscience and Nanotechnology Centre, Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital, India.
Objectives: The aim of this study was to investigate the potential of poly(amido amine) (PAMAM) dendrimer decorated graphene oxide (GO) based nanocarrier for targeted delivery of a hydrophobic anticancer drug, quercetin (QSR).
Methods: GO-PAMAM was successfully synthesized by covalent bonding between GO and NH2-terminated PAMAM dendrimer (zero generation). To investigate drug loading performance, QSR was loaded on the surface of GO as well as GO-PAMAM.
Charge shielding effects of PEG bound to NH-terminated PAMAM dendrimers - an experimental approach.
Soft Matter
April 2023
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.
Cationic poly(amido amine) (PAMAM) dendrimers exhibit great potential for use in drug delivery, but their high charge density leads to an inherent cytotoxicity. To increase biocompatibility, many studies have attached poly(ethylene glycol) (PEG) chains to the dendrimer surface. It is unclear how these tethered PEG chains influence the physicochemical properties of the dendrimer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!