Actin waves that travel on the planar membrane of a substrate-attached cell underscore the capability of the actin system to assemble into dynamic structures by the recruitment of proteins from the cytoplasm. The waves have no fixed shape, can reverse their direction of propagation and can fuse or divide. Actin waves separate two phases of the plasma membrane that are distinguished by their lipid composition. The area circumscribed by a wave resembles in its phosphoinositide content the interior of a phagocytic cup, leading us to explore the possibility that actin waves are in-plane phagocytic structures generated without the localized stimulus of an attached particle. Consistent with this view, wave-forming cells were found to exhibit a high propensity for taking up particles. Cells fed rod-shaped particles produced elongated phagocytic cups that displayed a zonal pattern that reflected in detail the actin and lipid pattern of free-running actin waves. Neutrophils and macrophages are known to spread on surfaces decorated with immune complexes, a process that has been interpreted as "frustrated" phagocytosis. We suggest that actin waves enable a phagocyte to scan a surface for particles that might be engulfed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802751 | PMC |
| http://dx.doi.org/10.4161/cam.3.4.9708 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Large, recursive membrane platforms are associated to Trop-1, Trop-2 and protein kinase signaling for cell growth.
Mol Biol Cell
January 2025
Laboratory of Cancer Pathology, Centre for Advanced Studies and Technology (CAST), University "G. D'Annunzio", Chieti, Italy.
The transmembrane glycoproteins Trop-1/EpCAM and Trop-2 independently trigger Ca and kinase signals for cell growth and tumor progression. Our findings indicated that Trop-1 and Trop-2 tightly colocalize at macroscopic, ruffle-like protrusions (RLP), that elevate from the cell perimeter, and locally recur over hundreds of seconds. These previously unrecognized elevated membrane regions ≥20 µm-long, up to 1.
View Article and Find Full Text PDFMembrane oscillations driven by Arp2/3 constrict the intercellular bridge during neural stem cell divisions.
bioRxiv
October 2024
Institute of Molecular Biology, Department of Chemistry and Biochemistry, 1229 University of Oregon, Eugene, OR 97403.
After the first furrowing step of animal cell division, the nascent sibling cells remain connected by a thin intercellular bridge (ICB). In isolated cells nascent siblings migrate away from each other to generate tension and constrict the ICB, but less is known about how cells complete cytokinesis when constrained within tissues. We examined the ICBs formed by larval brain neural stem cell (NSC) asymmetric divisions and find that they rely on constriction focused at the central midbody region rather than the flanking arms of isolated cell ICBs.
View Article and Find Full Text PDFUltrasound-activated microbubbles mediate F-actin disruptions and endothelial gap formation during sonoporation.
J Control Release
December 2024
Biomedical Engineering, Dept. of Cardiology, Cardiovascular Institute, Erasmus MC, Rotterdam, the Netherlands. Electronic address:
Locally opening up the endothelial barrier in a safe and controlled way is beneficial for drug delivery into the extravascular tissue. Although ultrasound-induced microbubble oscillations can affect the endothelial barrier integrity, the mechanism remains unknown. Here we uncover a new role for F-actin in microbubble-mediated endothelial gap formation.
View Article and Find Full Text PDFComparative Analyses of Dynamic Transcriptome Profile of Heart Highlight the Key Response Genes for Heat Stress in Zhikong Scallop .
Antioxidants (Basel)
October 2024
MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Heat stress resulting from global climate change has been demonstrated to adversely affect growth, development, and reproduction of marine organisms. The Zhikong scallop (), an important economical mollusk in China, faces increasing risks of summer mortality due to the prolonged heat waves. The heart, responsible for transporting gas and nutrients, is vital in maintaining homeostasis and physiological status in response to environmental changes.
View Article and Find Full Text PDFReproductive effort affects cellular response in the mantle of Nodipecten subnodosus scallops exposed to acute hyperthermia.
Comp Biochem Physiol A Mol Integr Physiol
January 2025
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, La Paz 23096, B.C.S, Mexico. Electronic address:
Article Synopsis
- Temperature impacts energy metabolism in marine ectotherms, leading to mass mortality during high reproductive energy demands.
- The study focused on the mantle of the scallop N. subnodosus to understand how it responds to acute hyperthermia during different reproductive stages (late gametogenesis in spring and ripe gonads in summer).
- Results showed varied cellular responses: spring scallops enhanced metabolic activity to maintain energy levels, while summer scallops exhibited reduced energy reserves and increased anaerobic metabolism, indicating seasonal adaptations to thermal stress.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!