A new HPLC system is described wherein the column is used in the form of a cassette. The system includes a novel, problem-free column connection technique (needle seal). The column is not fitted with the usual single inlet and single outlet, but with a system of four combined inlets/outlets. These features provide on the one hand simple operation (e.g., rapid column exchange--no tools needed) and increased performance (e.g., no loss of separating power in the ancillary equipment), and on the other hand, a high degree of flexibility (e.g., convenient column backflush, improved sample splitting, simple eluate splitting, elegant post-column derivatization, and easy column switching).
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| http://dx.doi.org/10.1093/chromsci/17.5.289 | DOI Listing |
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