Separation characteristics of animal cells using a dielectrophoretic filter.

Bioprocess Biosyst Eng

Division of Environment Management, Gunma University, Kiryu, Gunma, Japan.

Published: June 2010

AI Article Synopsis

  • The study evaluated a wire-wire type dielectrophoretic (DEP) filter's ability to separate animal cells based on their activity.
  • The results showed that cells retained in the DEP filter had a higher specific growth rate compared to those that permeated through it.
  • Additionally, the separation process resulted in a maximum difference of about 20% in retention ratios between the two groups of separated cell suspensions.

Article Abstract

The separation characteristics of a wire-wire type dielectrophoretic (DEP) filter were evaluated using animal cells. The separation of cells with different activities was examined using a DEP filter. The specific growth rate of the cells in retention liquid was larger than that in permeation liquid. From the culture results of the separated cells, it becomes clear that the specific growth rate of the cells of the retention liquid was higher than that of the cells of the permeation liquid. Furthermore, as a result of separating cells two kinds of cell suspensions using the DEP filter, the difference between the retention ratios of the two groups of obtained cells was about 20% at maximum.

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Source
http://dx.doi.org/10.1007/s00449-009-0382-1DOI Listing

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