We describe a wide-field interferometric surface-plasmon microscope capable of submicrometer resolution. The system is a speckle-illuminated Linnik interferometer, which behaves as a wide-field analog of a scanning heterodyne interferometer. The presented images demonstrate contrast reversals at different defocus while retaining submicrometer lateral resolution. The contrast mechanisms are discussed as well as the instrumental requirements of the technique.
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http://dx.doi.org/10.1364/OL.34.003110 | DOI Listing |
Single-shot 3D optical microscopy that can capture high-resolution information over a large volume has broad applications in biology. Existing 3D imaging methods using point-spread-function (PSF) engineering often have limited depth of field (DOF) or require custom and often complex design of phase masks. We propose a new, to the best of our knowledge, PSF approach that is easy to implement and offers a large DOF.
View Article and Find Full Text PDFFourier ptychographic microscopy (FPM) enables high-resolution, wide-field imaging of both amplitude and phase, presenting significant potential for applications in digital pathology and cell biology. However, artifacts commonly observed at the boundaries of reconstructed images can significantly degrade imaging quality and phase retrieval accuracy. These boundary artifacts are typically attributed to the use of the fast Fourier transform (FFT) on non-periodic images.
View Article and Find Full Text PDFTransl Vis Sci Technol
December 2024
Department of Ophthalmology & Vision Sciences, University of Toronto, Toronto, ON, Canada.
Purpose: To demonstrate that high-seed, ultra-high-resolution spectral-domain optical coherence tomography (SD-OCT) technology can image in vivo fine morphological features in the healthy and pathological human limbus.
Methods: A compact, fiberoptic SD-OCT system was developed for imaging the human limbus. It combines ∼1.
Nanophotonics
July 2024
School of Electronics and Information Engineering, Sichuan University, Chengdu 610065, China.
BMC Oral Health
December 2024
Oral and maxillofacial surgery, Faculty of Dentistry, University of Khartoum, Khartoum, Sudan.
Background: The DNA ploidy and the S phase fraction (SPF) are associated with advanced tumors progression. The present study aimed to investigate the clinicopathological behavior of oral squamous cell carcinoma(OSCC) and the prognostication of the DNA ploidy and the SPF on its clinical outcomes.
Methods: This a prospective longitudinal study of 93 OSCC patients (186 specimens).
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