AI Article Synopsis
- Aggregating brain cell cultures are 3D cultures made from embryonal rat brain cells.
- After 3-4 weeks of continuous agitation, these cultures develop structures and functions similar to real brain tissue.
- Stopping the agitation temporarily causes negative effects, similar to what happens in a brain experiencing ischemia, making this system a useful model for studying ischemia-related brain issues.
Article Abstract
Aggregating brain cell cultures are three-dimensional primary cell cultures derived from embryonal rat brain cells. Within 3-4 weeks in culture under continuous agitation, they exhibit organotypic structures and functions. The transient arrest of agitation induced adverse effects comparable with those occurring in the ischemic brain in vivo. This culture system therefore offers a suitable in vitro model for the elucidation of ischemia-related pathogenic processes in the brain.
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