Monozygotic multiple gestation after intracytoplasmic sperm injection and preimplantation genetic diagnosis.

Objective: To report a possible association between intracytoplasmic sperm injection (ICSI)-preimplantation genetic diagnosis (PGD) and monozygotic multiple gestation.

Design: Small case series.

Setting: In vitro fertilization unit in an academic medical center.

Patient(s): Three patients were treated with ICSI-PGD for sexing as well as selection against a known translocation.

Intervention(s): Transfer of day 4 embryos to the uterus.

Main Outcome Measure(s): Clinical pregnancy.

Result(s): Two pairs of monozygotic twins and a triplet pregnancy.

Conclusion(s): Repeated manipulation of the zona pellucida as well as extended embryo culture during ICSI-PGD treatments may result in monozygotic twin and triplet pregnancies.