AI Article Synopsis
- Dendritic cells (DC) are key players in the immune response and are used in vaccine development, with traditional methods taking around 8 days to produce them.
- Researchers developed faster protocols for generating DCs, evaluating their effectiveness through FACS analysis, where the optimal characteristics were established.
- Results indicate that DCs produced in just 5 or 48 hours show similar qualities to those made with the standard method, but 48-hour DCs are more sensitive to certain stimuli; further clinical testing is recommended.
Article Abstract
Dendritic cells (DC) are potent antigen presenting cells capable of inducing immune responses. DC are widely used as vaccine adjuvant in experimental clinical settings. DC-based vaccines are normally generated using a standard 8day DC protocol (SDDC). In attempts to shorten the vaccine production we have developed fast DC protocol by comparing two different fast DC protocols with SDDC. DC were evaluated by FACS analysis, and the optimal profile was considered: CD14(low), CD80(high), CD83(high), CD86(high), CCR7(high), HLA class I and II(high). FACS profiles were used as the selection criteria together with yield and morphology. Two fast DC protocols fulfilled these criteria and were selected for functional analysis. Our results demonstrate that DC generated within 5days or 48h are comparable with SDDC both phenotypically and functionally. However, we found that 48h DC were more susceptible than SDDC to the IL-10 inducing stimulus of TLR ligands (R848 and LPS). Thus to determine the clinical relevance of fast DC protocols in cancer settings, small phase I trials should be conducted monitoring regulatory T cells carefully.
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| http://dx.doi.org/10.1016/j.cellimm.2009.09.003 | DOI Listing |
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