Detection of group B Streptococcus (GBS) strains at various bacterial concentrations was evaluated using three pigment-producing broth media. At 10(3) CFU/ml, StrepB carrot broth (SBCB), Granada instant liquid biphasic (IGLB), and Northeast Laboratory GBS screening medium (NEL-GBS) showed 100% detection, but at the lower bacterial counts, SBCB and IGLB were more sensitive than NEL-GBS after 24 h.
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http://dx.doi.org/10.1128/JCM.01374-09 | DOI Listing |
Iran J Biotechnol
July 2024
Department of Plant Protection, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Background: is the causal agent of Fusarium Head Blight (FHB) on wheat and produces deoxynivalenol (DON), known to cause extreme human and animal toxicosis. This species' genome contains genes involved in plant-pathogen interactions and regulated by chromatin modifications. Moreover, histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), have been employed to study gene transcription regulation because they can convert the structure of chromatin.
View Article and Find Full Text PDFSci Total Environ
December 2024
Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Universidad Nacional de Salta (UNSa), Argentina; Facultad de Ciencias Naturales, UNSa, Argentina. Electronic address:
In this study, five strains previously isolated from black liquor (BL) and vinasse (V) were tested to assess the most promising regarding its capacity of biosurfactant production. For that, four factorial designs of two factors at two levels (2) were run for each strain. Selected factors were the production time and the composition media, while the surface tension reduction and optical density were the responses variables.
View Article and Find Full Text PDFJ Microbiol Methods
December 2024
Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, UK; UK Health Security Agency, Bacteriology Reference Department, London, UK. Electronic address:
Currently there is no detailed, internationally agreed protocol defined to evaluate antimicrobial susceptibility testing (AST) for Legionella pneumophila (required to establish epidemiological cut-off value or "ECOFF" boundaries); therefore, antimicrobial resistance in these isolates cannot be defined. AST methods utilising media containing activated charcoal as an ingredient, to enable Legionella growth, are unreliable as noted in an internationally authored opinion paper and a new gold standard is required. Here we define a detailed protocol for broth microdilution (BMD) using defined cell culture collection-deposited control reference strains (Philadelphia-1 and Knoxville-1) as well as two accessible reference strains with moderately (lpeAB-carrying) and markedly (23S rRNA mutation-carrying) elevated azithromycin minimum inhibitory concentration (MIC).
View Article and Find Full Text PDFAnal Biochem
December 2024
Biochemistry Department, University of Otago, Dunedin, New Zealand. Electronic address:
Staphylococcus aureus is a significant human pathogen causing acute life-threatening, and chronic infections often linked to biofilms. This study conducted a comparative lipidomic analysis of a methicillin-resistant (MRSA) and a methicillin-susceptible (MSSA) S. aureus strain in both planktonic and biofilm cultures using liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
View Article and Find Full Text PDFPol J Microbiol
December 2024
Department of Biology, Faculty of Science, Umm Al-Qura University, Makkah, Saudi Arabia.
spp. is a major source of global gastrointestinal infections. Their enteric infections are linked to the consumption of undercooked poultry products, contaminated milk and water, and the handling of wild animals and birds.
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