Lamina propria T lymphocytes (LPL-T) have a low proliferative potential in vitro. We asked whether LPL-T are also hyporesponsive in vivo and whether this is specific for the alphabeta T cell receptor (TCR). Mitogenic mAb directed at the alphabeta TCR, CD2, CD28, or control mAbs plus IL-2 were injected into rats. Proliferation and/or apoptosis were detected by double staining using 5-bromo-2'-deoxyuridine/TUNEL and the alphabeta TCR. LPL-T were hyporesponsive to various stimuli compared to other T cells. The strongest proliferation was found upon CD2/CD28 stimulation (LPL-T: 281 +/- 6%; spleen: 642 +/- 31%). LPL-T proliferation was only detectable at 24 h while proliferation in other compartments also occurred later. Hyporesponsiveness was not caused by enhanced T cell apoptosis upon alphabeta TCR stimulation. In conclusion, stimulation of LPL-T results in much shorter and weaker in vivo proliferation than in other lymphoid organs. Overall, CD2/CD28 costimulation is the strongest T cell stimulus in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/08820130902888342 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Human γδ T cells in the tumor microenvironment: Key insights for advancing cancer immunotherapy.
Mol Cells
January 2025
Laboratory of Host Defenses, Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea; KAIST Institute of Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Electronic address:
The role of γδ T cells in antitumor responses has gained significant attention due to their unique major histocompatibility complex (MHC)-independent killing mechanisms, which distinguish them from conventional αβ T cells. Notably, γδ tumor-infiltrating lymphocytes (TILs) have been identified as favorable prognostic markers in various cancers. However, γδ TIL subsets, including Vδ1, Vδ2, and Vδ3, exhibit distinct prognostic implications and phenotypes from one another within the tumor microenvironment (TME).
View Article and Find Full Text PDFStructural characterization of two γδ TCR/CD3 complexes.
Nat Commun
January 2025
Regeneron Pharmaceuticals, Inc., Tarrytown, NY, 10591, USA.
The T-cell receptor (TCR)/CD3 complex plays an essential role in the immune response and is a key player in cancer immunotherapies. There are two classes of TCR/CD3 complexes, defined by their TCR chain usage (αβ or γδ). Recently reported structures have revealed the organization of the αβ TCR/CD3 complex, but similar studies regarding the γδ TCR/CD3 complex have lagged behind.
View Article and Find Full Text PDFCharacterization of Human CD8αβ Interaction With Classical and Unconventional MHC Molecules.
Eur J Immunol
December 2024
Immunocore Limited, Abingdon, Oxon, UK.
The CD8 co-receptor exists as both an αα homodimer, expressed on subsets of specialized lymphoid cells, and as an αβ heterodimer, which is the canonical co-receptor on cytotoxic T-cells, tuning TCR thymic selection and antigen-reactivity in the periphery. However, the biophysical parameters governing human CD8αβ interactions with classical MHC class I (MHCI) and unconventional MHC-like molecules have not been determined. Using hetero-dimerized Fc-fusions to generate soluble human CD8αβ, we demonstrate similar weak binding affinity to multiple different MHCI alleles compared with CD8αα.
View Article and Find Full Text PDFSLAM/SAP signaling regulates discrete γδ T cell developmental checkpoints and shapes the innate-like γδ TCR repertoire.
Elife
December 2024
Department of Surgery, Larner College of Medicine, University of Vermont, Burlington, United States.
During thymic development, most γδ T cells acquire innate-like characteristics that are critical for their function in tumor surveillance, infectious disease, and tissue repair. The mechanisms, however, that regulate γδ T cell developmental programming remain unclear. Recently, we demonstrated that the SLAM/SAP signaling pathway regulates the development and function of multiple innate-like γδ T cell subsets.
View Article and Find Full Text PDFArticle Synopsis
- γδ T-cells play a crucial role in immune surveillance following HLA-Haploidentical Stem Cell Transplantation (haplo-HSCT), especially in pediatric patients.
- A study showed that a specific subset of Vδ2 T-cells is associated with better antiviral protection, as these cells were more prevalent in patients who did not experience viral reactivation.
- The research highlights how Vδ2 T-cells can inhibit CMV replication and enhance the immune response, suggesting their potential use in immunotherapy post-transplantation to combat both infections and tumors.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!