AI Article Synopsis
- The study compared four pretreatment methods for analyzing diclazuril and toltrazuril residues in chicken tissues, finding that all methods achieved an average recovery of 70%.
- The matrix solid-phase dispersion (MSPD) method was highlighted for its efficiency, saving over 60% in time and solvent compared to other methods.
- An MSPD-high performance liquid chromatography with ultraviolet detection (MSPD-HPLC/UV) was established, demonstrating good recovery rates (71.13% - 84.02%) and low detection limits (less than 10 microg/kg) for both chemicals, confirming the method's accuracy and precision for residue analysis.
Article Abstract
The effects of four pretreatment methods (acetonitrile extraction-evaporation concentration, acetonitrile extraction-solid phase extraction (SPE), matrix solid-phase dispersion (MSPD) extraction and MSPD-SPE) for the simultaneous analysis of diclazuril and toltrazuril residues in chicken tissues were compared. The average recovery of 70% for the former three methods as achieved. In comparison with other methods, the MSPD method saved more than 60% in time and solvent. So, MSPD as the sample pretreatment method, an MSPD-high performance liquid chromatography with ultraviolet detection (MSPD-HPLC/UV) method was established for the analysis. Under the optimal chromatographic conditions, the linear range was between 50 and 1,000 microg/kg. At the added levels of 50, 500, 1,000 ng/g, the recoveries of diclazuril and toltrazuril in chicken tissues ranged from 71.13% - 84.02% with the relative standard deviations (RSD) in the range of 3.76% - 12.11%, and the RSDs of intra- and interday analyses ranged from 3.70% - 6.77%. The detection limits of diclazuril and toltrazuril were less than 10 microg/kg. The quantitative limits of diclazuril and toltrazuril were less than 20 microg/kg. The method meet the requirements of the residue analysis on accuracy and precision.
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