AI Article Synopsis
- - Multi-drug-resistant bacteria pose significant treatment challenges, making bacterial efflux pumps a key target for new antibacterial strategies.
- - Resazurin, a compound used to track bacterial growth, is influenced by efflux pumps in Escherichia coli and Pseudomonas aeruginosa, which was demonstrated through flow cytometric analysis.
- - An assay to detect efflux pump activity was developed based on the resazurin to resorufin conversion rate, successfully identifying mefloquine as an efflux pump inhibitor during compound testing.
Article Abstract
Multi-drug-resistant bacteria can cause serious infections that are extremely difficult to treat. Bacterial efflux pumps are known to contribute to multi-drug resistance and, thus, constitute a promising target for novel antibacterial agents. Resazurin is widely used to monitor bacterial growth because resazurin is reduced to the fluorescent resorufin by live cells. We have shown by flow cytometric analysis and by accumulation studies with wild type and efflux deficient strains that resazurin is a substrate of efflux pumps in Escherichia coli and Pseudomonas aeruginosa. Our investigations showed that the conversion rate of resazurin to resorufin is affected by efflux pumps. This finding was used to design an assay useful to detect efflux pump activity and to find potential efflux-pump inhibitors in a microtiter plate format. Mefloquine was detected as efflux-pump inhibitor when a panel of selected chemical compounds was tested for assay validation purposes.
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| http://dx.doi.org/10.1016/j.mimet.2009.09.021 | DOI Listing |
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