AI Article Synopsis
- - The study examines how silencing the Annexin II (AnxA2) gene using siRNA affects the growth and invasiveness of Jurkat lymphoma cells.
- - Researchers transfected Jurkat cells with siRNA targeting AnxA2, measuring transfection success with real-time PCR and flow cytometry, followed by assessing cell proliferation and invasion through MTT assays and transwell plates.
- - Results showed that AnxA2 silencing significantly reduced the proliferation and invasive ability of Jurkat cells over time compared to control cells, suggesting a potential therapeutic target in lymphoma treatment.
Article Abstract
Objective: To investigate the effects of Annexin II (AnxA2) gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells.
Methods: A synthesized siRNA duplex targeting to AnxA2 was transfected into Jurkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTT assay for cell proliferation and transwell plates for invasive potential were performed.
Results: Compared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA transfected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [(17.4 +/- 2.3)%, (22.4 +/- 3.8)%, (37.6 +/- 1.5)% vs (-1.3 +/- 5.1)%, (-5.5 +/- 4.4)%, (-10.8 +/- 5.5)%, respectively, P<0.05]. The cell invasive potential of the transfected Jurkat cells was inhibited remarkably at 48 h (11.3 +/- 4.2 vs 54.3 +/- 8.7, P<0.01).
Conclusion: AnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.
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