AI Article Synopsis
- The study investigates how glutamine affects liver injury caused by LPS and D-Gal in Wistar rats.
- A total of 61 rats were divided into three groups to assess hepatic injury through various biochemical tests and microscopic examinations.
- Results indicated that glutamine pre-treatment did not significantly improve liver or intestinal health compared to the model group, despite lower apoptosis in hepatocytes in the glutamine group.
Article Abstract
Objective: To explore the role of glutamine in LPS and D-Gal induced acute hepatic injury.
Methods: A total of 61 Wistar rats were randomly divided into three groups: control group, model group and GLN pretreated group. The animal model was established by LPS and D-Gal intraperitoneal injection. GLN at dose of 1 g/kg was intragastrically administrated for 7 d before intraperitoneal injection. To evaluate the hepatic injury, the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL) were detected by automatic biochemistry analysator. The liver and bowel tissue was observed by lightmicroscope and transmission electron microscope (TEM). The apoptosis of hepatocyte was detected by TUNEL. HPLC-PED was used in the study of intestinal permeability.
Results: No significant differences were noted between ALT, AST, TBIL level, death rate and intestinal permeability (L/M) between model group and GLN pretreated group; In microscope, the confused structure of hepatic injury and inflammatory infiltration were similar between model group and GLN pretreated group. The injury of bowel was not obviously. Compared with the model group, there was better trend in liver and bowel in GLN pretreated group by transmission electron microscope (TEM). The apoptosis index in GLN pretreated group were lower than those in model group.
Conclusion: LPS can induce acute liver injury in D-Gal-sensitized rats.Glutamine has't the trend of protecting liver function and intestinal barrier function,decreasing death rates.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Non-thermal atmospheric pressure plasma (NTP), an ionized gas containing electrons, ions, radicals, and photons, has various biological effects, including wound healing and anticancer effects. Plasma-activated medium (PAM), which is prepared by irradiating medium with NTP, preferentially kills cancer cells. Large amounts of reactive oxygen species (ROS) and reactive nitrogen species (RNS) included in PAM are closely related to its anticancer effects.
View Article and Find Full Text PDFInsight into the herbicide resistance patterns in populations in Tunisian and Moroccan wheat regions.
Front Plant Sci
February 2024
Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czechia.
Article Synopsis
- A study examined 19 rigid ryegrass populations in Moroccan and Tunisian wheat fields to analyze their resistance to herbicides that inhibit acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase).
- Greenhouse experiments revealed all populations exhibited reduced sensitivity to these herbicides, with genetic testing confirming specific mutations linked to target-site resistance (TSR) in both ALS and ACCase enzyme genes.
- The research also identified non-target-site resistance (NTSR) in some populations, highlighting the need for effective local resistance management strategies due to the combined mechanisms of resistance present in these weed populations.
Annexin-A1 short peptide alleviates septic myocardial injury by upregulating SIRT3 and inhibiting myocardial cell apoptosis.
Histol Histopathol
July 2024
Department of Critical Care Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, PR China.
Septic myocardial injury is a common complication of severe sepsis, which occurs in about 50% of cases. Patients with this disease may experience varying degrees of myocardial damage. Annexin-A1 short peptide (ANXA1sp), with a molecular structure of Ac-Gln-Ala-Tyr, has been reported to exert an organ protective effect in the perioperative period by modulating sirtuin-3 (SIRT3).
View Article and Find Full Text PDFNeuroprotective effect of ketamine and sevoflurane against TNF-α induced cognitive impairment.
Environ Toxicol
March 2024
Internal Medicine-Neurology, Huanggang Central Hospital, Huanggang City, Hubei Province, China.
In the present study, neuroprotective effect of sevoflurane in combination with ketamine was investigated on TNF-α induced necroptosis of neurons and cognitive impairment in the rat model. The results demonstrated that exposure to TNF-α/z-VAD led to a significant decrease in viability of HT-22 neuronal cells. However, incubation of HT-22 cells with ketamine plus sevoflurane inhibited decrease in viability induced by TNF-α/z-VAD exposure.
View Article and Find Full Text PDFScreening and Constructing of Novel Angiotensin I-Converting Enzyme Inhibiting Peptides from Walnut Protein Isolate and Their Mechanisms of Action: A Merged In Silico and In Vitro Study.
Plant Foods Hum Nutr
March 2024
State Key Laboratory of Efficient Production of Forest Resources, Beijing Key Laboratory of Forest Processing and Safety, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Tsinghua East Road, Haidian District, Beijing City, 100083, China.
Article Synopsis
- Ultrasonic-assisted extraction was used to isolate ACE-inhibiting peptides from walnut protein isolate, with optimal conditions set at 15 minutes and 400 W.
- Two new peptides, Tyr-Ile-Gln (YIQ) and Ile-Tyr-Gln (IYQ), were identified, alongside a novel synthetic peptide, Ile-Lys-Gln (IKQ), which showed enhanced ACE-inhibiting activity.
- The study suggests these peptides could be non-toxic and beneficial for nitric oxide production, highlighting the potential of ultrasonicated walnut protein as a source for developing functional food ingredients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!