The aim of this study was to investigate the expression of progesterone receptor (PR) in the utero-tubal junction (UTJ) of sows at 24 h after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared with conventional artificial insemination (AI) in pigs. Fifteen multiparous sows were used: AI (n = 5), IUI (n = 5) and DIUI (n = 5). The sows were inseminated with a single dose of diluted semen during the second oestrus after weaning at 6-8 h prior to ovulation (AI: 3000 × 10(6) spermatozoa, IUI: 1000 × 10(6) spermatozoa and DIUI: 150 × 10(6) spermatozoa). The UTJ was collected and subject to immunohistochemical staining using avidin-biotin immunoperoxidase technique with mouse monoclonal antibody to PR. In the oviductal part of the UTJ, the intensity of PR in the tunica muscularis and the proportion of PR-positive cells in the surface epithelium after DIUI were lower than AI (p < 0.05). The intensity and the proportion of PR-positive cells between AI and IUI in all compartments of the UTJ did not differ significantly (p > 0.05). When comparing between tissue compartments, prominent staining was observed in the muscular layer of the UTJ. It could be concluded that the expression of PR in the UTJ prior to fertilization after DIUI with a reduced number of spermatozoa was lower than that after AI. This might influence sperm transportation and the fertilization process.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1439-0531.2009.01513.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
No impact of a positive Chlamydia trachomatis serology on live-birth rate after intra-uterine insemination.
J Gynecol Obstet Hum Reprod
January 2025
Department of Gynecology-Obstetric and Reproductive Medicine, AP-HM, La Conception University Hospital, Aix Marseille Univ, Marseille, France; IMBE, CNRS, IRD, Avignon Univ, Marseille, France.
Objective: To study the impact of a CT serology on intrauterine insemination (IUI) cumulative live-birth rate (cLBR) in women with documented bilateral tubal patency.
Design: Cohort study SUBJECTS: Infertile women with documented bilateral tubal patency and medical indication of IUI matched on the following criteria: woman's age, number of cycles completed and number of motile sperm inseminated (NMSI).
Exposure: This retrospective, observational and monocentric cohort study compared women with positive CT serology matched 1:1 to control women with negative CT serology.
Sperm morphology by strict criteria does not predict clinical pregnancy rate following intra-uterine insemination.
Arab J Urol
March 2024
Department of Infertility, Fakih IVF Fertility Center, Dubai, United Arab Emirates.
Objective: To determine the impact of abnormal sperm morphology of the pre-washed semen sample on the day of intrauterine insemination (IUI) on clinical pregnancy rates (CPR).
Design: Cross sectional retrospective chart review.
Setting: Academic fertility center.
Improving IVF Utilization with Patient-Centric Artificial Intelligence-Machine Learning (AI/ML): A Retrospective Multicenter Experience.
J Clin Med
June 2024
Atlantic Reproductive Medicine, Raleigh, NC 27617, USA.
In vitro fertilization (IVF) has the potential to give babies to millions more people globally, yet it continues to be underutilized. We established a globally applicable and locally adaptable IVF prognostics report and framework to support patient-provider counseling and enable validated, data-driven treatment decisions. This study investigates the IVF utilization rates associated with the usage of machine learning, center-specific (MLCS) prognostic reports (the Univfy report) in provider-patient pre-treatment and IVF counseling.
View Article and Find Full Text PDFSow reproductive performance following artificial insemination with semen doses processed using Single Layer Centrifugation without antibiotics in the tropics.
Theriogenology
September 2024
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Swine Reproduction, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:
Single Layer Centrifugation (SLC) through a low density colloid offers an alternative solution to antibiotic use in boar semen extenders, with lower costs compared to high density colloids. The aim of this study was to explore the reproductive performance of sows when using SLC-prepared semen doses without antibiotics, employing low density Porcicoll to prepare semen doses for artificial insemination in a commercial swine herd in Thailand. Ejaculates were divided into two equal parts to create insemination doses, with each dose containing 3000 × 10 sperm/80 ml for intra-uterine insemination in individual sows.
View Article and Find Full Text PDFPrognosis-based management of unexplained infertility-why not?
Hum Reprod Open
March 2024
Aberdeen Centre for Women's Health Research, Institute of Applied Health Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK.
Up to a half of couples seeking medical assistance for infertility are diagnosed with unexplained infertility, characterized by normal ovulation, tubal patency, and semen analysis results. This condition presents a challenge in determining the optimal treatment approach. Available treatments include IUI and IVF, but guidelines vary on when to offer each.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!