In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2786345 | PMC |
| http://dx.doi.org/10.1128/AAC.00803-09 | DOI Listing |
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