AI Article Synopsis
- Two Lactobacillus plantarum bacteriophages, FAGK1 and FAGK2, were isolated from Kefir grains, both sharing similar characteristics and belonging to the Siphoviridae family.
- They exhibited almost identical behavior in terms of host range, with only one strain showing different phage reactivity, and demonstrated a latent period of 30 minutes and burst periods of around 80 minutes, with an average burst size of 11 PFU per cell.
- This research is significant as it's the first time these specific bacteriophages have been isolated from Kefir grains, enhancing our understanding of the diverse microbial ecosystem in this fermented milk product.
Article Abstract
Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80+/-10 min and burst size values of 11.0+/-1.0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32.9, 35.7, 43.0 and 66.2 kDa). This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.
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| http://dx.doi.org/10.1017/S0022029909990203 | DOI Listing |
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