Previous studies have demonstrated that diospyrin (1), a quinonoid plant product, can inhibit the growth of Leishmania donovani parasites. Here, several derivatives of 1 were evaluated by the MTT assay and it was observed that the ethanolamine analogue (10) exhibited maximum cytotoxicity [50% inhibitory concentration (IC(50))=2.9 microM] against L. donovani promastigotes. Subsequently, the mode of cell death in promastigotes was investigated through externalisation of membrane-associated phosphatidylserine, mitochondrial membrane depolarisation, DNA laddering and in situ labelling of DNA fragmentation by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) methods. Whilst both 1 and 10 were found to induce apoptosis-like death in promastigotes, the effect of 10 was evidently stronger even at a lower concentration. Hence, the ethanolamine derivative (10) of diospyrin (1) may be a prospective 'lead' for the development of novel cytotoxic agents inducing apoptosis in L. donovani parasites.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijantimicag.2009.08.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High dose of ascorbic acid induces selective cell growth inhibition and cell death in human gastric signet-ring cell carcinoma-derived NUGC-4 cells.
Biochim Biophys Acta Gen Subj
February 2025
Program in Biological System Sciences Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, 5562, Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
Anticancer effects of high-dose vitamin C (VC) have been evaluated on many cancer cell lines, and its efficacy in clinical trials and in combination with anticancer drugs or radiation have been reported; however, its effect on gastric cancer and its mechanisms remain unclear. In the present study, the cell growth inhibitory/lethal effects of high-dose ascorbic acid (AsA), a reduced form of VC was examined on three gastric cancer cell lines. Of these, signet ring cell carcinoma NUGC-4 cells were the most sensitive, but the effects were small and limited in normal cells.
View Article and Find Full Text PDFVariable cyanobacterial death modes caused by ciprofloxacin in the aquatic environment: Prioritizing antibiotic-photosynthetic protein interactions for risk assessment.
Water Res
March 2025
Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, PR China; Key Laboratory of Microorganism Application and Risk Control, Ministry of Ecology and Environment, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, PR China; Shenzhen Key Laboratory of Ecological Remediation and Carbon Sequestration, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, PR China; Tsinghua University-Kunming Joint Research Center for Dianchi Plateau Lake, Tsinghua University, Beijing 100084, PR China. Electronic address:
Article Synopsis
- * At lower concentrations (3 μg/L), CIP primarily targets the PSII D1 protein, which leads to cell death through apoptosis-like mechanisms, while at higher concentrations (8 μg/L), it additionally affects PSI proteins, causing a switch to a different cell death pathway.
- * The study reveals how different antibiotic levels can change their lethal effects on photosynthetic organisms, highlighting the need for better risk assessment practices regarding the impact of antibiotics in aquatic environments.
Fungal Apoptosis-Related Proteins.
Microorganisms
November 2024
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Programmed cell death (PCD) plays a crucial role in the development and homeostasis maintenance of multicellular organisms. Apoptosis is a form of PCD that prevents pathological development by eliminating damaged or useless cells. Despite the complexity of fungal apoptosis mechanisms being similar to those of plants and metazoans, fungal apoptosis lacks the core regulatory elements of animal apoptosis.
View Article and Find Full Text PDFEnhanced suppression effects on Microcystis aeruginosa by combining hydrogen peroxide and intermittent UVC irradiation: The importance of triggering advanced oxidation process within cells.
J Hazard Mater
October 2023
Groundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China; Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China; Shenzhen Key Laboratory of Ecological Remediation and Carbon Sequestration, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China. Electronic address:
Article Synopsis
- * The first UVC treatment allows HO to enter algal cells, initiating an advanced oxidation process that gets activated with the second round of UVC, causing significant cellular damage.
- * This intermittent UVC/HO treatment disrupts the photosynthetic electron transport chain more effectively than conventional methods, leading to increased production of reactive oxygen species (ROS) that trigger cell death, making it a promising eco-friendly approach to control harmful algal blooms.
Clinical and molecular response to alpha1-oleate treatment in patients with bladder cancer.
Cancer Med
September 2024
Division of Microbiology, Immunology and Glycobiology, Department of Laboratory Medicine, Faculty of Medicine, Lund University, Sweden.
Background: The tumoricidal complex alpha1-oleate targets bladder cancer cells, triggering rapid, apoptosis-like tumor cell death. Clinical effects of alpha1-oleate were recently observed in patients with non-muscle invasive bladder cancer (NMIBC), using a randomized, placebo-controlled study protocol.
Aims: To investigate if there are dose-dependent effects of alpha1-oleate.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!