AI Article Synopsis
- The study aimed to explore how both natural (endogenous) and added (exogenous) hydrogen sulfide (H2S) affect potassium (K(ATP)) currents in isolated heart cells from rats.
- Using a whole-cell patch-clamp technique, the experiment measured the K(ATP) current density, revealing that an inhibitor of H2S (PPG) significantly reduced this current, while the H2S donor (NaHS) increased it in a dose-dependent manner.
- The findings suggest that H2S plays a crucial role in regulating K(ATP) channels, enhancing their currents in rat ventricular myocytes.
Article Abstract
Objective: To investigate the effects of endogenous and exogenous hydrogen sulfide (H(2)S) on the K(ATP) current in isolated rat ventricular myocytes.
Methods: Ventricular myocytes were isolated from rat heart by modified Langendorff perfusion with collagenase. K(ATP) current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique.
Results: The density of K(ATP) current was significantly reduced by 200 micromol/L DL-propargylglycine (PPG, an irreversible inhibitor of the H(2)S) [(5.3258 +/- 0.7556) pA/pF vs. (3.7856 +/- 0.4312) pA/pF, P < 0.01] in a time-dependent way. The density of K(ATP) current could be significantly increased by NaHS (a H(2)S donor, 9.375, 18.75, 37.5, 75, 150 micromol/L) in a concentration-dependent manner [(6.6310 +/- 0.6092) pA/pF vs. (9.0949 +/- 1.0259) pA/pF at 150 micromol/L, P < 0.01].
Conclusion: Both endogenous and exogenous H(2)S could open K(ATP) channels and enhance the K(ATP) current in rat ventricular myocytes.
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