The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764478 | PMC |
| http://dx.doi.org/10.1261/rna.1670909 | DOI Listing |
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