Objective: To improve the yield and quality of Dioscrea zingibiernsis.

Methods: The Biochemical indices and the diosgenin's content were analysed in the autotetraploid lines.

Results: The results showed that the activities of APX, SOD and POD in most of autotetraploid lines were higher than that in diploid line or close to it, there was also difference between autotetraploid lines and control lines in the SDS-PAGE of soluble proteins. The content of diosgenin in most autotetraploid plantlets were higher than that in the control.

Conclusion: There were difference between autotetraploid and control lines in the content of diosgenin and Biochemical indices, therefore, inducing autotetraploid could be an effective way to breeding the superior varieties.

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