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Determination of serum estradiol levels by radiometric and chemiluminescent techniques. | LitMetric

AI Article Synopsis

  • The measurement of hormone levels, particularly estradiol, is essential for successful management in assisted reproductive technologies like IVF.
  • Traditional methods like radioimmunoassay are being replaced by newer, nonradioactive techniques such as ELISA and chemiluminescent assays.
  • The chapter discusses the procedures for analyzing circulating estradiol levels using both radioimmunoassay and chemiluminescent methods.

Article Abstract

The ability to precisely measure circulating levels of hormones is a foundation of modern endocrinology. For assisted reproductive technologies such as in vitro fertilization (IVF), accurate determination of circulating levels of estradiol are crucial for patient management, retrieval of fertilizable oocytes, and successful pregnancy outcome. For many years, circulating levels of estradiol were determined by radioimmunoassay. More recently, nonradioactive techniques such as ELISAs or chemiluminescent approaches have replaced traditional radioimmunoassays. In the current chapter, we outline the procedures for analysis of circulating levels of estradiol by both radioimmunoassay and chemiluminescent techniques.

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Source
http://dx.doi.org/10.1007/978-1-60327-378-7_2DOI Listing

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