AI Article Synopsis
- The study investigated the link between early subjective responses like "dizziness" and genetic variations in CHRN genes among nicotine-dependent smokers and controls.
- Several SNPs in the CHRNB3 gene's promoter region showed a nominal association with the dizziness response, suggesting genetic factors may influence early reactions to smoking.
- Additionally, significant associations were found with SNPs in the CHRNA10 gene, highlighting its role in the early subjective experience of tobacco use.
Article Abstract
The neuronal nicotinic receptor genes (CHRN) have been implicated in a variety of smoking-related behaviors. Here we tested for association between an early subjective response phenotype, "dizziness," and 226 single nucleotide polymorphisms (SNPs) in CHRN genes. The sample included 789 nicotine-dependent cases and 811 controls, where early "dizziness" reports were significantly associated with case/control status (P < 0.0001). Multiple SNPs in the putative promoter region of the CHRNB3 gene were nominally associated with "dizziness" experience from the first few cigarettes (P < 0.01). Cell culture studies were conducted to examine the ability of different haplotypes in the CHRNB3 promoter to drive luciferase expression. Data from these experiments support the hypothesis that different alleles in the CHRNB3 upstream promoter region may lead to different levels of RNA expression. In addition, a novel finding of association between SNPs in the CHRNA10 gene reached experiment-wide empirical significance (P = 0.048), which implicates another CHRN gene as being involved in early subjective response to tobacco.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878135 | PMC |
| http://dx.doi.org/10.1002/ajmg.b.31027 | DOI Listing |
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