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Antimicrobial Peptides Against Arboviruses: Mechanisms, Challenges, and Future Directions.
Probiotics Antimicrob Proteins
January 2025
Department of Virology, Pasteur Institute of Iran, Tehran, 13169-43551, Iran.
This review delves into the potential of antimicrobial peptides (AMPs) as promising candidates for combating arboviruses, focusing on their mechanisms of antiviral activity, challenges, and future directions. AMPs have shown promise in preventing arbovirus attachment to host cells, inducing interferon production, and targeting multiple viral stages, illustrating their multifaceted impact on arbovirus infections. Structural elucidation of AMP-viral complexes is explored to deepen the understanding of molecular determinants governing viral neutralization, paving the way for structure-guided design.
View Article and Find Full Text PDFDevelopment of a dual-mode lateral flow assay based on structure-guided aptamers for the detection of capsaicin in gutter oils.
Biosens Bioelectron
March 2025
Teaching and Research Office of Food Safety, School of Public Course, Bengbu Medical College, Bengbu, 233000, China. Electronic address:
The construction of structure-guided aptamers and the ultra-sensitive aptamer-based lateral flow assays (Apt-LFA) integrated detection method hold significant potential for food analysis. Using an engineered modified sequence strategy, we successfully developed the aptamer Cap-1-2M4, significantly enhancing its affinity for capsaicin (CAP) to 0.6197 ± 0.
View Article and Find Full Text PDFSurface engineering of amine transaminases to control their region-selective immobilization.
Int J Biol Macromol
December 2024
Center for cooperative Research in Biomaterials (CIC biomaGUNE) - Basque Research and Technology Alliance (BRTA), Paseo de Miramón, 182, 20014 Donostia-San Sebastián, Spain; Ikerbasque, Basque Foundation for Science, Plaza Euskadi 5, 48009 Bilbao, Spain. Electronic address:
The industrial use of enzymes often requires their immobilization to facilitate downstream processing and enable reuse. However, controlling enzyme orientation during immobilization is challenging and typically restricted to the N- and C-terminal regions. In this work, we propose a strategy to immobilize more active and stable amine transaminases (ATAs) by combining protein engineering with immobilization techniques.
View Article and Find Full Text PDFCryo-EM structures of the full-length human contactin-2.
FEBS J
December 2024
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.
Contactin-2 (CNTN2), an immunoglobulin cell adhesion molecule (IgCAM) expressed on the neural cell surface, regulates the formation of myelin sheaths, facilitates communication between neurons and axoglial cells, and coordinates the migration of neural cells. However, the assembly of full-length CNTN2 is still not fully elucidated. Here, we found that the full-length human CNTN2 forms a concentration-dependent homodimer.
View Article and Find Full Text PDFStructure-guided engineering an (R)-transaminase from Mycobacterium neoaurum for efficient synthesis of chiral N-heterocyclic amines.
Int J Biol Macromol
December 2024
School of Life Sciences and Biopharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe, Shenyang 110016, People's Republic of China. Electronic address:
(R)-selective amine transaminases (R-ATAs) show considerable potential for the asymmetric synthesis of chiral drug intermediates. However, the low catalytic efficiency of natural R-ATAs toward bulky ketone substrates, such as N-heterocyclic compounds, severely limits its industrial application. In this study, five putative (R)-ATAs were mined from NCBI database, among which MnTA showed the highest activity for N-Boc-3-pyrrolidinone (1a) and N-Boc-3-piperidone (2a), and its crystal structure was performed.
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