[Effects and mechanism of decorin on the proliferation of HuH7 hepatoma carcinoma cells in vitro].

Aim: To investigate the effects and mechanism of decorin( DCN) on the proliferation of HuH7 hepatoma carcinoma cell line in vitro.

Methods: Hepatoma carcinoma cells was cultured with DCN in different concentration (0, 25, 50, 75, 100, 125, 150, 200 microg/L) for different time(12, 24, 48, 72 h and 2 weeks). Cell activities were studied by MTT and clone test. The changes of cell cycle and apoptosis were analyzed by Flow cytometry.

Results: The proliferation of HuH7 cells could be inhibited by DCN in vitro and the inhibition effect was the time and dose dependent relationship. DCN could block cell cycle at G(1); phase. Apoptosis of hepatocarcinoma cells could be efficiently induced by DCN in a time/dose-dependent manner.

Conclusion: DCN may be a negative regulatory protein inhibiting hepatoma carcinoma cell proliferation through inhibiting cell cycle and inducing apoptosis of cell in vitro.