AI Article Synopsis
- F1-ATPase is a motor protein that can produce ATP by reversing its rotary motion, impacting how it facilitates catalytic reactions.
- Research shows that the rate at which F1 binds ATP increases when the rotor rotates forward, suggesting that rotation enhances its binding affinity for ATP.
- This study provides new insights into how the rotational angle of F1 influences its catalytic activity and ATP production.
Article Abstract
F1-ATPase (F1) is a reversible ATP-driven rotary motor protein. When its rotary shaft is reversely rotated, F1 produces ATP against the chemical potential of ATP hydrolysis, suggesting that F1 modulates the rate constants and equilibriums of catalytic reaction steps depending on the rotary angle of the shaft. Although the chemomechanical coupling scheme of F1 has been determined, it is unclear how individual catalytic reaction steps depend on its rotary angle. Here, we report direct evidence that the ATP-binding rate of F1 increases upon the forward rotation of the rotor, and its binding affinity to ATP is enhanced by rotation.
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| http://dx.doi.org/10.1016/j.febslet.2009.08.042 | DOI Listing |
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