Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells.

Opt Express

Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University Durham, North Carolina 27708, USA.

Published: August 2009

We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microscopy of live human skin cancer cells.

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.17.015585DOI Listing

Publication Analysis

Top Keywords

detector bandwidth
8
microscopy live
8
two-step-only phase-shifting
4
interferometry
4
phase-shifting interferometry
4
interferometry optimized
4
optimized detector
4
bandwidth microscopy
4
live cells
4
cells phase-shifting
4

Similar Publications

High-Brightness Color-Tunable AC-Driven Quantum Dot Light-Emitting Diodes for Integrated Passive High-Electric-Field Contactless Detection.

ACS Appl Mater Interfaces

December 2024

Institute of Optoelectronics Technology, Key Laboratory of Luminescence and Optical Information, Beijing Jiaotong University, Beijing 100044, China.

This work explores the carrier recombination dynamics of AC-driven quantum dot (QD) light-emitting diodes (AC-QLEDs) and proposes their application in the field of electric field contactless detection. Different sequences of green QD (GQD)/red QD (RQD) bilayer thin films as the emission layer of AC-QLEDs were fabricated via film transfer printing to ensure the complete morphology of each layer. AC-QLEDs with the emission layer as the sequence of GQD + RQD (GR-QLEDs) show a significantly enhanced carrier recombination efficiency due to its stable energy level structure, achieving the highest peak brightness ever recorded for vertically emitting brightness of 1648.

View Article and Find Full Text PDF

O-band intensity modulation and direct detection (IM/DD) transmission offers a promising solution for high-speed data center interconnects (DCIs). Additionally, the introduction of bismuth-doped fiber amplifiers (BDFAs) results in less nonlinear impact and a higher link budget compared with semiconductor optical amplifiers (SOAs). However, with these key issues resolved, the system bandwidth limitation emerges as the next critical bottleneck for high-speed O-band DCI transmission that limits overall performance.

View Article and Find Full Text PDF

A novel high frequency, high bandwidth, three phase Mach-Zehnder optical data link.

Rev Sci Instrum

December 2024

Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, New Mexico 87545, USA.

In inertial confinement fusion, hydrogen isotopes are fused together under high pressures and temperatures. Typically, the duration of these experiments is incredibly short, on the order of around 60-150 ps. Due to the high radiation environment, a detector's signal is typically data linked far distances to a protected location.

View Article and Find Full Text PDF
Article Synopsis
  • The rise of modern telecommunication technologies, like 5G and the Internet-of-Things (IoT), has significantly increased data generation and the demand for advanced data processing capabilities.
  • Researchers introduced a microwave photonic (MWP) processing unit that uses elemental antimony to create a compact all-optical RF filter designed to operate as a low-pass filter with a bandwidth of 300 kHz.
  • The study demonstrates the filter's use as an envelope detector for demodulating signals and discusses potential methods for achieving tunable bandwidth.
View Article and Find Full Text PDF

[Analysis on Spatial-temporal Characteristics and Driving Factors of PM in Heilongjiang Province in the Past 20 years].

Huan Jing Ke Xue

December 2024

Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China.

PM is an important indicator for measuring the degree of air pollution. Studying the space-time variation and the driving factors of spatial heterogeneity is important for controlling air pollution and improving regional air quality. Based on PM remote sensing data from 2000 to 2021, the Theil-Sen Median trend analysis, Mann-Kendall significant inspection, and spatial auto correlation were used to analyze the characteristics of space-time changes in PM concentration, and geographical detectors were combined with a multi-scale geographical weighted regression model to explore the key driver factor and its influence and direction of the impact and role of PM spatial differences.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!