Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1292/jvms.71.1133 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing.
J Vet Med Sci
December 2024
Laboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University.
A comprehensive and quantitative method to compare gene expression may be useful for investigating the mechanisms responsible for diminazene aceturate (DA) resistance in Babesia gibsoni. Therefore, the gene expression of B. gibsoni cultured with DA was compared with those without DA using RNA sequencing (RNA-seq).
View Article and Find Full Text PDFTick-, flea- and mite-borne pathogens and associated diseases of public health importance in Bangladesh: a review.
Infect Med (Beijing)
December 2024
Jiann-Ping Hsu College of Public Health, Georgia Southern University, Statesboro, GA 30458, USA.
Background: This scoping review provides a baseline summary of the current records of the ticks, fleas, and mites of public health importance that are present in Bangladesh. It summarizes their geographic distributions and reports the levels of their infestation of livestock, pets, wildlife, and humans, and the clinical and epidemiological studies pertinent to these vectors and their pathogens.
Methods: Sixty-one articles were identified in a literature search, including 43 published since 2011.
Preassembled complexes of hAgo2 and ssRNA delivered by nanoparticles: a novel silencing gene expression approach overcoming the absence of the canonical pathway of siRNA processing in the apicomplexan parasite Babesia microti, blood parasite of veterinary and zoonotic importance.
Emerg Microbes Infect
December 2025
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Article Synopsis
- Current chemical drugs are ineffective for tick control and there are no effective vaccines, leading to the urgent need for new control strategies.
- Small interfering RNAs (siRNAs) have potential to help control parasites, but their effectiveness is limited by challenges in getting them to the RNA-induced silencing complex (RISC).
- This study developed a delivery system using chitosan nanoparticles to successfully transfect siRNAs into parasites, which reduced target gene expression and inhibited parasite growth, opening new avenues for gene therapy and understanding host-parasite interactions.
Exploring tick-borne pathogens in community dogs in Nepal.
Parasitol Int
November 2024
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Japan; Division of Parasitology, Veterinary Research Unit, International Institute for Zoonosis Control, Hokkaido University, Japan. Electronic address:
Article Synopsis
- Tick-borne pathogens (TBPs) in dogs pose a significant global health threat, particularly in developing countries where surveillance is lacking.
- A study involving 230 community dogs in Nepal found that 32.6% were infected with at least one TBP, with common pathogens including Ehrlichia canis and Anaplasma platys.
- The findings suggest that infections in community dogs might pose a risk for transmission to pet dogs and humans, highlighting the need for increased awareness and control measures against these zoonotic parasites.
[Detection of Babesia spp. in ticks and in blood of dogs and red deer in the Czech Republic].
Epidemiol Mikrobiol Imunol
November 2024
Article Synopsis
- The study aimed to explore the presence of potentially harmful Babesia species in ticks and the blood of dogs and deer across different regions of the Czech Republic, comparing their prevalence with other tick-borne pathogens like Borrelia and Anaplasma.
- From 2014 to 2016, researchers analyzed 675 Ixodes ricinus ticks and blood samples from dogs and deer, finding low positivity rates for Babesia spp., with identified species including Babesia venatorum and Babesia microti, both of which can affect humans.
- The prevalence rates of Babesia in ticks were found to be lower than that of Borrelia spp. (29.3%) and comparable to Rickettsia spp. (1.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!