AI Article Synopsis
- A Real-time quantitative PCR assay was developed to specifically quantify the Toxoplasma gondii apicoplast using designed primers that amplify a 305bp DNA product.
- Standard curves for both apicoplast and genomic DNA were created, allowing researchers to measure the amount of apicoplast DNA in test samples.
- The study found that exposure to antibiotics like ciprofloxacin, clindamycin, and azithromycin significantly reduced the levels of T. gondii apicoplast DNA in the second infectious cycle, demonstrating the usefulness of Real-time PCR for testing anti-apicoplast drugs.
Article Abstract
A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicoplast DNA copies in the test samples. The results indicated that the copies of T. gondii apicoplast DNA was significantly low when exposed to ciprofloxacin, clindamycin and azithromycin for 48h in the second infectious cycle. Our study shows that the Real-time PCR technique is a simple and quick technique for screening the anti-apicoplast drugs.
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| http://dx.doi.org/10.1016/j.exppara.2009.08.013 | DOI Listing |
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