AI Article Synopsis
- Researchers identified a new anti-viral factor called X-DING-CD4 from HIV-1 resistant cells known as HRF(+) cells, which shares similarities with DING proteins.
- The factor was confirmed to be present in the extracellular space of HRF(+) cells using a specific antibody, differentiating it from HRF(-) control cells.
- X-DING-CD4 was shown to block HIV-1 replication and expression in certain assays, with its activity being neutralized by targeted antibodies.
Article Abstract
We reported previously the anti-viral activity named HRF (HIV-1 Resistance Factor) secreted by HIV-1 resistant cells. This work describes the identification of HRF from cell culture supernatant of HRF-producing cells (HRF(+) cells). Employing the proteomics and cell based activity assay we recovered ten peptides sharing 80-93% sequence homology with other eukaryotic DING proteins; discrete amino acid characteristics found in our material suggested that HRF is a new member of DING proteins family and consequently we designated it as X-DING-CD4 (extracellular DING from CD4(+) T cells). The presence of X-DING-CD4 in the extracellular compartment of HRF(+) but not control HRF(-) cells was confirmed by specific anti-X-DING-CD4 antibody. Similar as the un-fractionated HRF(+) cell culture supernatant, the purified X-DING-CD4 blocked transcription of HIV-1 LTR-promoted expression of luciferase gene and replication of HIV-1 in MAGI cells. The X-DING-CD4 -mediated anti-viral activity in MAGI cells could be blocked by specific antibody.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957897 | PMC |
| http://dx.doi.org/10.1016/j.bbrc.2009.08.140 | DOI Listing |
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