Recombinant protein production by a Kaiko-baculovirus system.

Methods Mol Biol

Katakura Industries Co., Ltd., Saitama, Japan.

Published: February 2010

Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).

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http://dx.doi.org/10.1007/978-1-60761-232-2_9DOI Listing

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