AI Article Synopsis
- ABA triggers reactive oxygen species (ROS) production in guard cells through NADPH oxidases AtrbohD and AtrbohF, leading to stomatal closure.
- The protein kinase OST1, activated by ABA, plays a key role upstream of ROS in the signaling pathway for guard cells.
- Research shows that OST1 phosphorylates specific serine residues on AtrbohF, affecting its activity, and that OST1 physically interacts with AtrbohF, providing evidence of regulation.
Article Abstract
The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a approximately 40% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.febslet.2009.08.033 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!