Investigating the role of intestinal microbial populations significantly relies on the assumption of stability. Therefore, the microbial community composition of the simulator of the human intestinal microbial ecosystem was qualitatively, quantitatively and functionally characterised during reactor start-up to evaluate its capacity to produce a stable bacterial community, representative for the human intestinal tract. Using moving window correlation, a stability criterion was introduced to analyse the stability over time of the PCR-DGGE, plate counts, short chain fatty acids and ammonium results. A community was regarded stable when minimum 80% correlation was measured over at least one cell residence time. Species composition stability was reached after about 2 weeks, while it took some 3 weeks to reach functional stability. The combination of PCR-DGGE with moving window correlation proved to be an efficient approach to quantitatively evaluate the stability of the in vitro cultured intestinal microbial community.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.femsec.2004.05.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Interaction of the Gut-Liver-Brain Axis and the sterolbiome with sexual dysfunction in patients with cirrhosis.
Gut Microbes
December 2025
Division of Gastroenterology, Hepatology, and Nutrition, Virginia Commonwealth University and Richmond VA Medical Center, Richmond, VA, USA.
There is a complex interplay between the gut microbes, liver, and central nervous system, a gut-liver-brain axis, where the brain impacts intestinal and hepatic function while the gut and liver can impact cognition and mental status. Dysregulation of this axis can be seen in numerous diseases. Hepatic encephalopathy, a consequence of cirrhosis, is perhaps the best studied perturbation of this system.
View Article and Find Full Text PDFAdditive effect of mixtures of multiple strains combining the effects of single strains on human gut microbiota .
Biosci Microbiota Food Health
August 2024
Central Research Institute, Itoen Ltd., 21 Mekami, Sagara-cho, Haibara-gun, Shizuoka, Japan.
Probiotics exert their beneficial effects by improving the intestinal environment. Heat-inactivated probiotics may show similar effects. However, whether multi-strain mixtures (MSM) are better than single strains, irrespective of whether the bacteria are alive or dead, is unknown.
View Article and Find Full Text PDFUnlabelled: The T cell receptor (TCR) repertoire of intestinal CD4+ T cells is enriched for specificity towards microbiome-encoded epitopes shared among many microbiome members, providing broad microbial reactivity from a limited pool of cells. These cells actively coordinate mutualistic host-microbiome interactions, yet many epitopes are shared between gut symbionts and closely related pathobionts and pathogens. Given the disparate impacts of these agents on host health, intestinal CD4+ T cells must maintain strain-level discriminatory power to ensure protective immunity while preventing inappropriate responses against symbionts.
View Article and Find Full Text PDFMaternal milk fat globule membrane enriched gut and circulating SCFAs to improve placental efficiency and fetal development in intrauterine growth restricted mice model.
Gut Microbes
December 2025
Department of Obstetrics and Gynecology and Reproductive Medicine, Peking University First Hospital, Beijing, China.
Intrauterine growth restriction (IUGR) caused by placental dysfunctions leads to fetal growth defects. Maternal microbiome and its metabolites have been reported to promote placental development. Milk fat globule membrane (MFGM) is known for its diverse bioactive functions, while the effects of gestational MFGM supplementation on the maternal gut microbiota, placental efficiency, and fetal development remained unclear.
View Article and Find Full Text PDFDivergence of gut bacteria through the selection of genomic variants implicated in the metabolism of sugars, amino acids, and purines by small extracellular vesicles in milk.
Gut Microbes
December 2025
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Here, we report that small extracellular vesicles (sEVs) in milk mediate the communication between bacteria and animal kingdoms, increase the divergence of bacteria in the intestine, and alter metabolite production by bacteria. We show that bovine milk sEVs select approximately 55,000 genomic variants in 19 species of bacteria from the murine cecum . The genomic variants are transcribed into mRNA.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!