AI Article Synopsis
- The nanos genes are crucial for developing primordial germ cells in animals, particularly in sea urchins like Hemicentrotus pulcherrimus, where HpNanos mRNA is specifically expressed in small micromere descendants.
- Morpholino knockdown of HpNanos leads to delayed cell movement and reduced cell numbers in the left coelomic pouch, impacting embryonic development.
- The study suggests that HpNanos plays a critical role in interactions between cell lineages and is necessary for the survival of small micromere descendants.
Article Abstract
The nanos genes play important roles in the development of primordial germ cells in animal species. In the sea urchin, Hemicentrotus pulcherrimus, small micromere descendants specifically express HpNanos mRNA and this expression continues in the left coelomic pouch, which produces the major component of the adult rudiment. In this study, we showed that morpholino knockdown of HpNanos resulted in a delay of primary mesenchyme cell ingression and a decrease in the number of cells comprising the left coelomic pouch. Knockdown analysis in chimeras and whole embryos revealed the disappearance of small micromere descendants from the archenteron tip. Furthermore, the expression of HpNanos mRNA was induced in other cell lineages in the HpNanos-knockdown and micromere-deleted embryos. Taken together, our results suggest that HpNanos is involved in the inductive interaction of small micromere descendants with other cell lineages, and that HpNanos is required for the survival of small micromere descendants.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/dvdy.22074 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Lineage tracing shows that cell size asymmetries predict the dorsoventral axis in the sea star embryo.
BMC Biol
August 2022
Center for Marine Biotechnology and Biomedicine, University of California San Diego, La Jolla, CA, 92093, USA.
Background: Cell size asymmetries are often linked to cell fate decisions, due to cell volumes and cell fate determinants being unequally partitioned during asymmetric cell divisions. A clear example is found in the sea urchin embryo, where a characteristic and obvious unequal 4th cleavage generates micromeres, which are necessary for mesendoderm cell fate specification. Unlike sea urchin development, sea star development is generally thought to have only equal cleavage.
View Article and Find Full Text PDFEmbryonic development in the acoel Hofstenia miamia.
Development
July 2021
Department of Organismic and Evolutionary Biology, Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA.
Acoels are marine worms that belong to the phylum Xenacoelomorpha, a deep-diverging bilaterian lineage. This makes acoels an attractive system for studying the evolution of major bilaterian traits. Thus far, acoel development has not been described in detail at the morphological and transcriptomic levels in a species in which functional genetic studies are possible.
View Article and Find Full Text PDFDistinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.
Dev Biol
August 2019
Department of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting Street, Providence, RI, 02912, USA. Electronic address:
Specification of the primordial germ cells (PGCs) is essential for sexually reproducing animals. Although the mechanisms of PGC specification are diverse between organisms, the RNA binding protein Nanos is consistently required in the germ line in all species tested. How Nanos is selectively expressed in the germ line, however, remains largely elusive.
View Article and Find Full Text PDFMethods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).
Methods Cell Biol
June 2019
Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI, United States.
Small micromeres of the sea urchin are believed to be primordial germ cells (PGCs), fated to give rise to sperm or eggs in the adult. Sea urchin PGCs are formed at the fifth cleavage, undergo one additional division during blastulation, and migrate to the coelomic pouches of the pluteus larva. The goal of this chapter is to detail classical and modern techniques used to analyze primordial germ cell specification, gene expression programs, and cell behaviors in fixed and live embryos.
View Article and Find Full Text PDFEarly development of the feeding larva of the sea urchin Heliocidaris tuberculata: role of the small micromeres.
Dev Genes Evol
January 2019
School of Medical Sciences F13 and School of Life and Environmental Sciences A11, University of Sydney, NSW, 2006, Australia.
The two modes of development in sea urchins are direct development, in which the adult develops directly from the gastrula to the adult and does not feed, and indirect development, in which the adult develops indirectly through a feeding larva. In this account of the indirect, feeding larva of Heliocidaris tuberculata, the question raised is whether an evolutionary difference of unequal cell divisions contributes to the development of feeding structures in the indirect larva. In indirect development, the cell divisions at the fourth and fifth cell cycles of the zygote are unequal, with four small micromeres formed at the vegetal pole at the fifth cell division.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!