Purpose: To quantify the intensity of the expression of neutral and acids mucins in mucosa of the colon with and without fecal stream and to correlate this with the duration of fecal transit diversion.
Methods: Thirty male Wistar rats were subjected to fecal transit deviation in the left colon by a proximal colostomy and a distal mucous fistula. The animals were divided into three experimental groups, according to whether sacrificing would be performed six, 12 or 18 weeks after surgery. The expression of neutral and acid mucins was evaluated using the histochemical techniques of Periodic Acid Schiff and Alcian Blue, respectively. The tissue mucins expression was quantified by computer-assisted image analysis software (NIS-Elements) in the segments with and without fecal stream. Student's paired t test was used to compare the quantities of mucins in colon with or without fecal stream and variance between the experimental groups by ANOVA and Newman-Keuls post-test, establishing level of signification of 5% (p<0.05).
Results: There were significant decreased quantities of acid and neutral mucins in the colon without transit, compared with the colon with fecal stream, independent of the duration of exclusion. There was increased expression of neutral mucins in the colon with fecal stream after 12 and 18 weeks of exclusion. There was no increase in the expression of acid mucins in the colon with transit as the duration of fecal transit exclusion progressed. There was increased production of acid mucins in the animals submitted to diversion of the fecal stream for 18 weeks, compared with those subjected to diversion for 6 and 12 weeks. In the colon without fecal stream, there was increased expression of neutral mucins after 12 and 18 weeks of exclusion.
Conclusions: Deviation of the fecal stream decreased the expression of acid and neutral mucins in the segments without fecal transit, compared with segments with transit. Regardless of the reduced expression of acid and neutral mucins in the segments without fecal stream, their tissue expression increased with increasing duration of intestinal deviation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1590/s0102-86502009000400005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of viable Enterobacteriaceae and Enterococcus spp. across the agro-ecosystem of an antibiotic-free swine farm.
Can J Microbiol
January 2025
Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec, Canada;
Agricultural practices, specifically the use of antibiotics and other biocides, have repercussions on human, animal and plant health. The aim of this study was to evaluate the levels of Enterobacteriaceae and Enterococcus, as antibiotic resistant marker bacteria, in various matrices across the agro-ecosystem of an antibiotic-free swine farm in Quebec (Canada), namely pig feed, feces, manure, agricultural soil, water and sediment from a crossing stream, and soil from nearby forests. Samples were collected in fall 2022, spring and fall 2023 and spring 2024.
View Article and Find Full Text PDFAssessment of Antibiotic Resistance Among Isolates of spp. and spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Outcome.
Pathogens
January 2025
MicroART-Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
One of the significant challenges facing modern medicine is the rising rate of antibiotic resistance, which impacts public health, animal health, and environmental preservation. Evaluating antibiotic resistance in wildlife and their environments is crucial, as it offers essential insights into the dynamics of resistance patterns and promotes strategies for monitoring, prevention, and intervention. and genera isolates were recovered from fecal samples of wild animals and environmental samples using media without antibiotic supplementation.
View Article and Find Full Text PDFCombined application of metagenomics and FEAST to trace sources of microbial eukaryotic contamination in the Pasig-Marikina-San Juan (PAMARISAN) river system in Metro Manila, Philippines.
Environ Monit Assess
January 2025
Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, 1101, Quezon City, Philippines.
Microbial eukaryotes are vital to global microbial diversity, but there is limited information about their composition and sources in contaminated surface waters. This study examined the pathogens and potential sources of microbial eukaryotic communities in polluted sink environments using the 18S rDNA amplicon sequencing combined with the fast expectation-maximization for microbial source tracking (FEAST) program. Six sampling sites were selected along the Pasig-Marikina-San Juan (PAMARISAN) River System, representing different locations within the waterway and classified as sinks (n = 12), whereas animal fecal samples collected from various farms were classified as sources (n = 29).
View Article and Find Full Text PDFRisks of parasitic helminth disease outbreaks and influence of physico-chemical factors in flood prone areas of Yaoundé, Cameroon.
Parasite Epidemiol Control
February 2025
Centre for Research on Health and Priority Pathologies, Institute of Medical Research and Medicinal Plants Studies, PO box 13033 Yaoundé, Cameroon.
Background: Urbanization coupled with poverty has promoted the exploitation of risk zones like flood-prone areas in the city of Yaoundé. The overcrowding and poor hygiene observed in these areas are responsible for the unsmiling variations in environmental cleanliness, exploitation of river water for domestic purposes thus putting them at risk for parasitic disease transmissions. This study was conducted in order to assess the risks of human helminthiases outbreaks in relation to water physico-chemical factors in the city of Yaoundé.
View Article and Find Full Text PDFLongitudinal metagenomic analysis on antibiotic resistome, mobilome, and microbiome of river ecosystems in a sub-tropical metropolitan city.
Water Res
January 2025
Environmental Microbiome Engineering and Biotechnology Laboratory, Center for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Hong Kong SAR, China; School of Public Health, The University of Hong Kong, Hong Kong SAR, China; State Key Laboratory of Marine Pollution, Department of Chemistry and School of Energy and Environment, City University of Hong Kong, Hong Kong SAR, China; Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao SAR, China. Electronic address:
Rivers play an important role as reservoirs and sinks for antibiotic resistance genes (ARGs). However, it remains underexplored for the resistome and associated mobilome in river ecosystems, and hosts of riverine ARGs particularly the pathogenic ones are rarely studied. This study for the first time conducted a longitudinal metagenomic analysis to unveil the resistome, mobilome, and microbiome in river water, by collecting samples from 16 rivers in Hong Kong over a three-year period and using both short-read and long-read sequencing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!