New reusable Celite/ethylene glycol cartridges for selective chromatography of steroids before immunoassay.

Objectives: Preparation of reusable and easy to handle Celite chromatographic columns.

Design And Methods: Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure.

Results: These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.

Conclusions: The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.