AI Article Synopsis
- Metabotropic glutamate (mGlu) receptors are important for how glutamate affects the central nervous system, with mGlu(5) receptor interacting with various proteins to regulate its function.
- Research showed that NECAB2, a protein, specifically interacts with the mGlu(5) receptor, and this interaction depends on calcium levels, meaning that higher calcium concentrations reduce their binding.
- The study demonstrated that NECAB2 and mGlu(5) are found together in the same part of brain cells, and their interaction influences important signaling pathways related to neuronal activity.
Article Abstract
Metabotropic glutamate (mGlu) receptors mediate in part the CNS effects of glutamate. These receptors interact with a large array of intracellular proteins in which the final role is to regulate receptor function. Here, using co-immunoprecipitation and pull-down experiments we showed a close and specific interaction between mGlu(5) receptor and NECAB2 in both transfected human embryonic kidney cells and rat hippocampus. Interestingly, in pull-down experiments increasing concentrations of calcium drastically reduced the ability of these two proteins to interact, suggesting that NECAB2 binds to mGlu(5) receptor in a calcium-regulated manner. Immunoelectron microscopy detection of NECAB2 and mGlu(5) receptor in the rat hippocampal formation indicated that both proteins are codistributed in the same subcellular compartment of pyramidal cells. In addition, the NECAB2/mGlu(5) receptor interaction regulated mGlu(5b)-mediated activation of both inositol phosphate accumulation and the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway. Overall, these findings indicate that NECAB2 by its physical interaction with mGlu(5b) receptor modulates receptor function.
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| http://dx.doi.org/10.1111/j.1471-4159.2009.06348.x | DOI Listing |
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