A single gene, AIN, in Medicago truncatula mediates a hypersensitive response to both bluegreen aphid and pea aphid, but confers resistance only to bluegreen aphid.

J Exp Bot

Commonwealth Scientific and Industrial Research Organisation Entomology, Private Bag 5, Wembley, WA 6913, Australia.

Published: February 2010

Biotic stress in plants frequently induces a hypersensitive response (HR). This distinctive reaction has been studied intensively in several pathosystems and has shed light on the biology of defence signalling. Compared with microbial pathogens, relatively little is known about the role of the HR in defence against insects. Reference genotype A17 of Medicago truncatula Gaertn., a model legume, responds to aphids of the genus Acyrthosiphon with necrotic lesions resembling a HR. In this study, the biochemical nature of this response, its mode of inheritance, and its relationship with defence against aphids were investigated. The necrotic lesion phenotype and resistance to the bluegreen aphid (BGA, Acyrthosiphon kondoi Shinji) and the pea aphid (PA, Acyrthosiphon pisum (Harris)) were analysed using reference genotypes A17 and A20, their F(2) progeny and recombinant inbred lines. BGA-induced necrotic lesions co-localized with the production of H(2)O(2), consistent with an oxidative burst widely associated with hypersensitivity. This HR correlated with stronger resistance to BGA in A17 than in A20; these phenotypes cosegregated as a semi-dominant gene, AIN (Acyrthosiphon-induced necrosis). In contrast to BGA, stronger resistance to PA in A17, compared with A20, did not cosegregate with a PA-induced HR. The AIN locus resides in a cluster of sequences predicted to encode the CC-NBS-LRR subfamily of resistance proteins. AIN-mediated resistance presents a novel opportunity to use a model plant and model aphid to study the role of the HR in defence responses to phloem-feeding insects.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755030PMC
http://dx.doi.org/10.1093/jxb/erp244DOI Listing

Publication Analysis

Top Keywords

bluegreen aphid
12
gene ain
8
medicago truncatula
8
hypersensitive response
8
pea aphid
8
resistance bluegreen
8
role defence
8
necrotic lesions
8
a17 a20
8
stronger resistance
8

Similar Publications

Background: The bluegreen aphid (Acyrthosiphon kondoi) is a worldwide pest of alfalfa, pulses, and other legume crops. An overreliance on insecticides to control A. kondoi has potentially placed populations under selection pressure favouring resistant phenotypes, but to date, there have been no documented cases of insecticide resistance.

View Article and Find Full Text PDF

Strongylodon macrobotrys: new host of soybean mosaic virus in Brazil.

Plant Dis

January 2021

Universidade de São Paulo - ESALQ, Fitopatologia e Nematologia, Av. Padua Dias 11, Piracicaba, São Paulo, Brazil, 13418-900;

Strongylodon macrobotrys, commonly known as the jade vine, emerald vine, or turquoise jade vine, is a species of Fabaceae native to the Philippines. The plants have blue-green color inflorescences, which makinge them one of the most admired ornamental plants in Brazil (Muniz et al. 2015).

View Article and Find Full Text PDF

Aphids are important agricultural pests causing major yield losses worldwide. Since aphids can rapidly develop resistance to chemical insecticides there is an urgent need to find alternative aphid pest management strategies. Despite the economic importance of bluegreen aphid (Acyrthosiphon kondoi), very few genetic resources are available to expand our current understanding and help find viable control solutions.

View Article and Find Full Text PDF

Gene pyramids and the balancing act of keeping pests at bay.

J Exp Bot

September 2019

Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Iowa, USA.

This article comments on: Kamphuis LG, Klingler JP, Jacques S, Gao L-l, Edwards OR, Singh KB. 2019. Additive and epistatic interactions between and loci conferring bluegreen aphid resistance and hypersensitivity in .

View Article and Find Full Text PDF

Aphids, including the bluegreen aphid (BGA; Acyrthosiphon kondoi), are important pests in agriculture. Two BGA resistance genes have been identified in the model legume Medicago truncatula, namely AKR (Acyrthosiphon kondoi resistance) and AIN (Acyrthosiphon induced necrosis). In this study, progeny derived from a cross between a resistant accession named Jester and a highly susceptible accession named A20 were used to study the interaction between the AKR and AIN loci with respect to BGA performance and plant response to BGA infestation.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!