The action of high-intensity laser u.v. radiation on nucleic acid molecules and their constituents in vitro and in vivo is compared with the results of low-intensity u.v. photolysis and gamma-radiolysis.
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http://dx.doi.org/10.1080/09553009014552411 | DOI Listing |
Phys Chem Chem Phys
January 2007
Max-Planck-Institut für Bioanorganische Chemie (formerly Max-Planck-Institut für Strahlenchemie), D-45413, Mülheim, Germany.
The time-resolved formation of the retinyl carbocation from all-trans-retinol and all-trans-retinol acetate was studied by use of picosecond flash photolysis. From both precursors, the retinyl cation is produced by heterolytic C-O bond cleavage in solvents of medium polarity (acetonitrile, tetrahydrofuran, propanol with Reichardt polarity parameter ET(N) approximately 0.5) and high polarity (EtOH, MeOH, TFE, HFIP, ET(N) > 0.
View Article and Find Full Text PDFPhotochem Photobiol Sci
October 2002
Max-Planck-lnstitut für Strahlenchemie, D-45413 Mülheim, Germany.
Time-resolved pico- and nanosecond transient absorption measurements of 8-methoxypsoralen (8-MOP) were performed in different solvents at room temperature. The excited singlet state has two strong absorption bands with maxima at 430 and 630-690 nm. The lifetime of the first excited singlet state, tau(s), ranges from <10 ps up to 2.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
February 2000
Institute of Chemical Physics of the Russian Academy of Sciences, Moscow.
A new approach to the theory of the EPR of spin correlated radical pairs (SCRP) is formulated. It is based on spectral exchange methods as applied to the two-site model and takes into account explicitly both the motion of one quantum (transverse magnetization) and two quantum coherences (polarizations) of SCRPs. This innovation allows for the interpretation of anti phase structure (APS) spectral shape asymmetry and for the transformation of the initially created ST0RPM polarization into the APS spectral pattern.
View Article and Find Full Text PDFThe efficiency of the photomodification of target single-stranded DNA with a decanucleotide derivative of p-azidotetrafluorobenzamide (direct photomodification) and with its complexes with decanucleotide derivatives of pyrene complementary to the adjacent segment of the target (sensitized photomodification) was studied as a function of the wavelength of long-wave UV light. The sensitized photomodification occurs mainly by singlet-singlet energy transfer from pyrene to azide in their complementary complex, which allows a significant increase in the rate and level of photomodification. When irradiation occurred simultaneously in the UV and visible regions (365-580 nm), two-photon triplet-triplet sensitization was revealed for the first time, which leads to a still greater acceleration of the target modification and a change of its site-direction from the G11 to T13 residue.
View Article and Find Full Text PDFJ Photochem Photobiol B
May 1990
Institute of Spectrocopy, U.S.S.R. Academy of Sciences, Troitzk, Moscow Region.
The method of high performance liquid chromatography was applied to investigate the products of single-quantum and two-quantum photo-reactions which occur when an aqueous solution of uridine is irradiated with high intensity nanosecond and picosecond laser UV pulses. A comparison of the experimental results obtained with theoretical models enables a number of photophysical parameters of the highly excited electronic states of the uridine molecule to be determined.
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