We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765152 | PMC |
| http://dx.doi.org/10.1128/AEM.01587-09 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Trehalose Interferes with the Photosynthetic Electron Transfer Chain of Permeating the Bacterial Chromatophore Membrane.
Int J Mol Sci
December 2024
Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio n.42, 40126 Bologna, Italy.
Disaccharide trehalose has been proven in many cases to be particularly effective in preserving the functional and structural integrity of biological macromolecules. In this work, we studied its effect on the electron transfer reactions that occur in the chromatophores of the photosynthetic bacterium . In the presence of a high concentration of trehalose, following the activation of the photochemistry by flashes of light, a slowdown of the electrogenic reactions related to the activity of the photosynthetic reaction center and cytochtome (cyt) complexes is observable.
View Article and Find Full Text PDFEnhancing Proton-Coupled Electron Transfer in Blue Light Using FAD Photoreceptor AppA.
J Am Chem Soc
January 2025
Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, United States.
The Blue Light Using FAD (BLUF) photoreceptor utilizes a noncovalently bound FAD to absorb light and trigger the initial ultrafast events in receptor activation. FAD undergoes 1 and 2 electron reduction as an enzyme redox cofactor, and studies on the BLUF photoreceptor PixD revealed the formation of flavin radicals (FAD and FADH) during the photocycle, supporting a general mechanism for BLUF operation that involves PCET from a conserved Tyr to the oxidized FAD. However, no radical intermediates are observed in the closely related BLUF proteins AppA and BlsA, and replacing the conserved Tyr with fluoro-Tyr analogs that increase the acidity of the phenol OH has a minor effect on AppA photoactivation in contrast to PixD where the photocycle is halted at FAD.
View Article and Find Full Text PDFThe potential correlations between cell-free extracts from grown under low-oxygen conditions and volatile organic compounds in Chinese-style sausage.
Food Chem X
December 2024
Meat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu Univeristy, Chengdu 610106, China.
Limited research has explored the use of cell-free extracts (RCFE) in meat processing. To examine the potential application of RCFE in improving the flavor quality of Chinese-style sausage, in this study, we investigated the effects and mechanisms of RCFE grown under low-oxygen conditions on the flavor development of Chinese-style sausage, using GC-MS and 4D label-free proteomics. The GC-MS analysis detected 60 volatile organic compounds, with significant increases in acids, esters, and alcohols following the addition of RCFE ( < 0.
View Article and Find Full Text PDFEngineering the Methylerythritol Phosphate Pathway and Using a Temporal Promoter for Enhanced Lycopene Production in HY01.
J Agric Food Chem
December 2024
State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
HY01 is a high-yield strain for industrial production of coenzyme Q (Q), indicating its potential for producing other terpenoids. However, the production of Q substantially depletes isoprene precursors, nearly eliminating other terpenoids like spheroidene and spheroidenone commonly found in wild-type . Lycopene was used as an example to demonstrate its potential for terpenoid biosynthesis.
View Article and Find Full Text PDFContribution of Protonation to the Dielectric Relaxation Arising from Bacteriopheophytin Reductions in the Photosynthetic Reaction Centers of .
Biomolecules
October 2024
Institute of Medical Physics, University of Szeged, 6720 Szeged, Hungary.
The pH dependence of the free energy level of the flash-induced primary charge pair PI was determined by a combination of the results from the indirect charge recombination of PQ and from the delayed fluorescence of the excited dimer (P*) in the reaction center of the photosynthetic bacterium , where the native ubiquinone at the primary quinone binding site Q was replaced by low-potential anthraquinone (AQ) derivatives. The following observations were made: (1) The free energy state of PI was pH independent below pH 10 (-370 ± 10 meV relative to that of the excited dimer P*) and showed a remarkable decrease (about 20 meV/pH unit) above pH 10. A part of the dielectric relaxation of the PI charge pair that is not insignificant (about 120 meV) should come from protonation-related changes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!