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Cohesin positions the epigenetic reader Phf2 within the genome.
EMBO J
January 2025
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030, Vienna, Austria.
Article Synopsis
- Genomic DNA is organized into chromatin with the help of histones and cohesin, but their cooperation in genome regulation is not well understood.
- Researchers identified Phf2, a histone demethylase, as a protein that interacts with cohesin, indicating a potential role in regulating transcription at active gene sites.
- The studies show that Phf2 helps recruit cohesin to transcription start sites and affects the size of chromatin compartments, highlighting an important relationship between histone modification and genome architecture in eukaryotic cells.
A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes.
bioRxiv
December 2024
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA USA.
The nucleosome remodeler Chd1 is required for the re-establishment of nucleosome positioning in the wake of transcription elongation by RNA Polymerase II. Previously, we found that Chd1 occupancy on gene bodies depends on the Rtf1 subunit of the Paf1 complex in yeast. Here, we identify an N-terminal region of Rtf1 and the CHCT domain of Chd1 as sufficient for their interaction and demonstrate that this interaction is direct.
View Article and Find Full Text PDFGenome-wide profiling and functional study of short N-terminal H2B variants in Arabidopsis.
J Adv Res
December 2024
Department of Urology, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; Hubei Hongshan Laboratory, Wuhan 430071, China. Electronic address:
Article Synopsis
- - The study investigates the roles of specific histone H2B variants in the plant Arabidopsis thaliana, focusing on how these variants influence chromatin localization and gene regulation, which are key to understanding epigenetic mechanisms in plants.
- - Four short N-terminal H2B variants (snH2Bs) were identified, and their genomic distribution, impact on plant growth, and regulatory functions were analyzed using advanced techniques like ChIP-seq combined with CRISPR-Cas9 gene editing.
- - Results show that these snH2B variants are mainly expressed in reproductive tissues and play significant roles in gene expression and the transition from vegetative to reproductive stages in plants, highlighting their importance in chromatin regulation.
Molecular Dynamics Simulations of Nucleosomes Containing Histone Variant H2A.J.
Int J Mol Sci
November 2024
Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Histone proteins form the building blocks of chromatin-nucleosomes. Incorporation of alternative histone variants instead of the major (canonical) histones into nucleosomes is a key mechanism enabling epigenetic regulation of genome functioning. In humans, H2A.
View Article and Find Full Text PDFCAF-1 promotes efficient PrimPol recruitment to nascent DNA for single-stranded DNA gap formation.
Nucleic Acids Res
December 2024
Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Article Synopsis
- - The accumulation of single-stranded DNA (ssDNA) gaps at replication forks may influence how sensitive certain tumors are to chemotherapy, particularly those deficient in homologous recombination (HR), since these ssDNA gaps can turn into harmful double-stranded DNA breaks.
- - The study highlights the crucial role of the histone chaperone CAF-1, not just in maintaining stalled replication forks, but also in promoting ssDNA gap formation via its interaction with PrimPol, an enzyme that aids in managing replication stress.
- - Findings suggest that the loss of CAF-1 or ASF1A in HR-deficient cells leads to increased chemoresistance due to a reduction in ssDNA gaps, pointing to a novel function of CAF-
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